嗜热栖热菌HB8葡萄糖脱氢酶的初步X射线晶体学研究。
Preliminary X-ray crystallographic study of glucose dehydrogenase from Thermus thermophilus HB8.
作者信息
Kanaujia Shankar Prasad, Ranjani Chellamuthu Vasuki, Jeyakanthan Jeyaraman, Nishida Masami, Kitamura Yoshiaki, Baba Seiki, Ebihara Akio, Shimizu Nobutaka, Nakagawa Noriko, Shinkai Akeo, Yamamoto Masaki, Kuramitsu Seiki, Shiro Yoshitsugu, Sekar Kanagaraj, Yokoyama Shigeyuki
机构信息
Bioinformatics Centre (Centre of Excellence in Structural Biology and Biocomputing), Indian Institute of Science, Bangalore 560 012, India.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 May 1;63(Pt 5):446-8. doi: 10.1107/S1744309107014819. Epub 2007 Apr 28.
Abstract
Thermus thermophilus is an aerobic chemoorganotroph that has been found to grow anaerobically in the presence of nitrate. Crystals of glucose dehydrogenase (GDH) from T. thermophilus HB8 belong to space group P2(1), with unit-cell parameters a = 36.90, b = 132.96, c = 60.78 A, beta = 97.2 degrees. Preliminary studies and molecular-replacement calculations reveal that the asymmetric unit contains two monomers.
摘要
嗜热栖热菌是一种需氧化学有机营养菌，已发现它在硝酸盐存在的情况下可进行厌氧生长。嗜热栖热菌HB8的葡萄糖脱氢酶（GDH）晶体属于空间群P2(1)，晶胞参数为a = 36.90、b = 132.96、c = 60.78 Å，β = 97.2°。初步研究和分子置换计算表明，不对称单元包含两个单体。
相似文献
1
Preliminary X-ray crystallographic study of glucose dehydrogenase from Thermus thermophilus HB8.嗜热栖热菌HB8葡萄糖脱氢酶的初步X射线晶体学研究。
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 May 1;63(Pt 5):446-8. doi: 10.1107/S1744309107014819. Epub 2007 Apr 28.
2
Cloning, expression, purification, crystallization and preliminary X-ray crystallographic study of molybdopterin synthase from Thermus thermophilus HB8.嗜热栖热菌HB8钼蝶呤合酶的克隆、表达、纯化、结晶及初步X射线晶体学研究
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Apr 1;63(Pt 4):324-6. doi: 10.1107/S1744309107011426. Epub 2007 Mar 30.
3
Cloning, expression, purification, crystallization and preliminary X-ray crystallographic study of thymidylate kinase (TTHA1607) from Thermus thermophilus HB8.嗜热栖热菌HB8胸苷酸激酶（TTHA1607）的克隆、表达、纯化、结晶及初步X射线晶体学研究
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Feb 1;69(Pt 2):118-21. doi: 10.1107/S1744309112050208. Epub 2013 Jan 30.
4
Crystallization and preliminary X-ray crystallographic studies of NADP-dependent 3-hydroxyisobutyrate dehydrogenase from Thermus thermophilus HB8.嗜热栖热菌HB8中依赖NADP的3-羟基异丁酸脱氢酶的结晶及初步X射线晶体学研究。
Acta Crystallogr D Biol Crystallogr. 2003 Dec;59(Pt 12):2294-6. doi: 10.1107/s090744490302002x. Epub 2003 Nov 27.
5
Crystallization and preliminary X-ray crystallographic studies of the small form of glucose-inhibited division protein A from Thermus thermophilus HB8.嗜热栖热菌HB8中葡萄糖抑制分裂蛋白A小形式的结晶及初步X射线晶体学研究。
Acta Crystallogr D Biol Crystallogr. 2004 Mar;60(Pt 3):515-7. doi: 10.1107/S0907444904000721. Epub 2004 Feb 25.
6
Cloning, expression, purification, crystallization and preliminary crystallographic analysis of the putative NlpC/P60 endopeptidase, TTHA0266, from Thermus thermophilus HB8.嗜热栖热菌HB8中假定的NlpC/P60内肽酶TTHA0266的克隆、表达、纯化、结晶及初步晶体学分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Nov;69(Pt 11):1291-4. doi: 10.1107/S1744309113027164. Epub 2013 Oct 30.
7
Crystallization and preliminary X-ray studies of isocitrate dehydrogenase from Thermus thermophilus HB8.嗜热栖热菌HB8异柠檬酸脱氢酶的结晶及初步X射线研究。
J Biochem. 1995 Oct;118(4):679-80. doi: 10.1093/oxfordjournals.jbchem.a124963.
8
Expression, purification and preliminary X-ray characterization of CTP synthetase from Thermus thermophilus HB8.嗜热栖热菌HB8中CTP合成酶的表达、纯化及初步X射线表征
Acta Crystallogr D Biol Crystallogr. 2003 Mar;59(Pt 3):551-3. doi: 10.1107/s0907444903000106. Epub 2003 Feb 21.
9
Crystallization and preliminary X-ray diffraction studies of a DNA excision repair enzyme, UvrB, from Thermus thermophilus HB8.嗜热栖热菌HB8来源的DNA切除修复酶UvrB的结晶及初步X射线衍射研究
Acta Crystallogr D Biol Crystallogr. 1999 Mar;55(Pt 3):704-5. doi: 10.1107/s0907444998015777.
10
Crystallization and preliminary X-ray crystallographic analysis of peptide deformylase from Thermus thermophilus HB8.嗜热栖热菌HB8肽脱甲酰基酶的结晶及初步X射线晶体学分析
Acta Crystallogr D Biol Crystallogr. 2004 Jul;60(Pt 7):1299-300. doi: 10.1107/S0907444904010595. Epub 2004 Jun 22.
本文引用的文献
1
Processing of X-ray diffraction data collected in oscillation mode.振荡模式下收集的X射线衍射数据的处理。
Methods Enzymol. 1997;276:307-26. doi: 10.1016/S0076-6879(97)76066-X.
2
Free R value: a novel statistical quantity for assessing the accuracy of crystal structures.自由R值：一种用于评估晶体结构准确性的新型统计量。
Nature. 1992 Jan 30;355(6359):472-5. doi: 10.1038/355472a0.
3
Soluble aldose sugar dehydrogenase from Escherichia coli: a highly exposed active site conferring broad substrate specificity.来自大肠杆菌的可溶性醛糖脱氢酶：具有广泛底物特异性的高度暴露活性位点。
J Biol Chem. 2006 Oct 13;281(41):30650-9. doi: 10.1074/jbc.M601783200. Epub 2006 Jul 24.
4
The structural basis for substrate promiscuity in 2-keto-3-deoxygluconate aldolase from the Entner-Doudoroff pathway in Sulfolobus solfataricus.嗜热栖热菌中Entner-Doudoroff途径的2-酮-3-脱氧葡萄糖酸醛缩酶底物选择性的结构基础。
J Biol Chem. 2004 Oct 15;279(42):43886-92. doi: 10.1074/jbc.M407702200. Epub 2004 Jul 20.
5
Biotechnologically relevant enzymes from Thermus thermophilus.来自嗜热栖热菌的与生物技术相关的酶。
Appl Microbiol Biotechnol. 2002 Jan;58(1):1-12. doi: 10.1007/s00253-001-0843-1.
6
Glucose transport in the extremely thermoacidophilic Sulfolobus solfataricus involves a high-affinity membrane-integrated binding protein.极端嗜热嗜酸的嗜热栖热菌中的葡萄糖转运涉及一种高亲和力的膜整合结合蛋白。
J Bacteriol. 1999 Jul;181(14):4285-91. doi: 10.1128/JB.181.14.4285-4291.1999.
7
Crystallography & NMR system: A new software suite for macromolecular structure determination.晶体学与核磁共振系统：用于大分子结构测定的新软件套件。
Acta Crystallogr D Biol Crystallogr. 1998 Sep 1;54(Pt 5):905-21. doi: 10.1107/s0907444998003254.
8
Anaerobic growth, a property horizontally transferred by an Hfr-like mechanism among extreme thermophiles.厌氧生长，一种通过类似高频重组（Hfr）机制在极端嗜热菌之间水平转移的特性。
J Bacteriol. 1998 Jun;180(12):3137-43. doi: 10.1128/JB.180.12.3137-3143.1998.
9
Bioenergetics of the archaebacterium Sulfolobus.古细菌嗜热栖热菌的生物能量学
Biochim Biophys Acta. 1996 Dec 18;1277(3):163-200. doi: 10.1016/s0005-2728(96)00104-1.
10
Solvent content of protein crystals.蛋白质晶体的溶剂含量。
J Mol Biol. 1968 Apr 28;33(2):491-7. doi: 10.1016/0022-2836(68)90205-2.
Zotero 插件