The Key Laboratory for Chemical Biology of Fujian Province, and Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China.
Analyst. 2012 Feb 7;137(3):710-5. doi: 10.1039/c1an15647k. Epub 2011 Dec 5.
Herein we propose a novel method for ultrasensitive detection of phenolic compounds. This method was developed based on a spin-labeled terbium complex Tb(3+)/cs124-DTPA-TEMPO (1). This spin-labeled terbium complex is a weakly luminescent compound and shows strong off-on luminescent response to phenolic compounds in the presence of horseradish peroxidase (HRP), glutathione (GSH) and hydrogen peroxide. The analyte recognition and signaling mechanism are discussed and the factors affecting the off-on luminescence have been explored. Detection limits of 1.1 nM for phenol, 1.1 nM for resorcine, 0.6 nM for m-cresol, 3 nM for p-cresol, and 0.5 nM for 2,4-dichlorophenol were obtained, respectively. The practicability of the proposed method has been tested in detection of the concentration of spiked nearshore seawaters, and recoveries of 77.4-80.4% with relative standard deviations (RSDs) of 1.0-2.2% were obtained.
本文提出了一种用于检测酚类化合物的新方法。该方法基于一种自旋标记的铽配合物 Tb(3+)/cs124-DTPA-TEMPO(1)。这种自旋标记的铽配合物是一种弱发光化合物，在辣根过氧化物酶(HRP)、谷胱甘肽(GSH)和过氧化氢存在下，对酚类化合物表现出强的关闭-开启发光响应。讨论了分析物识别和信号转导机制，并探讨了影响关闭-开启发光的因素。分别得到了对苯酚、间苯二酚、间甲酚、对甲酚和 2,4-二氯苯酚的检测限为 1.1 nM、1.1 nM、0.6 nM、3 nM 和 0.5 nM。该方法的实用性已在近岸海水的浓度检测中得到检验，回收率为 77.4-80.4%，相对标准偏差(RSD)为 1.0-2.2%。
