Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, 106 91 Stockholm, Sweden.
J Am Chem Soc. 2012 Jun 20;134(24):9832-5. doi: 10.1021/ja3004045. Epub 2012 Feb 29.
A new strategy for accessing analyte-responsive luminescent probes is presented. The lanthanide luminescence of Eu and Tb centers is switched on by the analyte-triggered formation of a sensitizing antenna from a nonsensitizing caged precursor. As the cage can be freely varied, an array of probes for different analytes (Pd(0/2+), H(2)O(2), F(-), β-galactosidase) can be created from the same core structure. The probe design affords nanomolar to micromolar detection limits, provides the capability to detect two analytes in parallel, and can be utilized to monitor enzymatic activity in live cells.
提出了一种新的分析物响应型荧光探针的检测策略。Eu 和 Tb 中心的镧系元素荧光通过分析物触发的从非敏化笼状前体形成敏化天线来打开。由于笼可以自由变化，因此可以从相同的核心结构创建用于不同分析物（Pd(0/2+)、H(2)O(2)、F(-)、β-半乳糖苷酶）的探针阵列。该探针设计具有纳摩尔至微摩尔的检测限，能够同时检测两种分析物，并可用于监测活细胞中的酶活性。
