Zscharnack Kristin, Kreisig Thomas, Prasse Agneta A, Zuchner Thole
Institute of Bioanalytical Chemistry, Faculty of Chemistry and Mineralogy, Universität Leipzig, Deutscher Platz 5, Leipzig 04103, Germany.
Institute of Bioanalytical Chemistry, Faculty of Chemistry and Mineralogy, Universität Leipzig, Deutscher Platz 5, Leipzig 04103, Germany; Center for Biotechnology and Biomedicine, Universität Leipzig, Deutscher Platz 5, Leipzig 04103, Germany.
Anal Chim Acta. 2014 Jun 27;834:51-7. doi: 10.1016/j.aca.2014.05.019. Epub 2014 May 17.
Here, we present a fast and simple hydrogen peroxide assay that is based on time-resolved fluorescence. The emission intensity of a complex consisting of terbium ions (Tb(3+)) and phthalic acid (PA) in HEPES buffer is quenched in the presence of H2O2 and this quenching is concentration-dependent. The novel PATb assay detects hydrogen peroxide at a pH range from 7.5 to 8.5 and with a detection limit of 150 nmol L(-1) at pH 8.5. The total assay time is less than 1 min. The linear range of the assay can be adapted by a pH adjustment of the aqueous buffer and covers a concentration range from 310 nmol L(-1) to 2.56 mmol L(-1) in total which encompasses four orders of magnitude. The assay is compatible with high concentrations of all 47 tested inorganic and organic compounds. The PATb assay was applied to quantify H2O2 in polluted river water samples. In conclusion, this fast and easy-to-use assay detects H2O2 with high sensitivity and precision.
在此，我们展示了一种基于时间分辨荧光的快速简便的过氧化氢检测方法。在HEPES缓冲液中，由铽离子（Tb(3+)）和邻苯二甲酸（PA）组成的复合物的发射强度在有过氧化氢存在时会被淬灭，且这种淬灭与浓度相关。这种新型的PATb检测方法在pH值7.5至8.5的范围内检测过氧化氢，在pH 8.5时检测限为150 nmol L(-1)。总检测时间不到1分钟。该检测方法的线性范围可通过调整水性缓冲液的pH值来调节，总共覆盖310 nmol L(-1)至2.56 mmol L(-1)的浓度范围，跨度达四个数量级。该检测方法与所有47种测试的无机和有机化合物的高浓度兼容。PATb检测方法被用于定量污染河流水样中的过氧化氢。总之，这种快速且易于使用的检测方法能够高灵敏度和高精度地检测过氧化氢。
