[世界卫生组织实验室手册第五版与第四版精子形态评估标准的比较研究]

[A comparative study of sperm morphology evaluation criteria by the fifth and fourth editions of WHO Laboratory Manual].

作者信息

Zhang Xin-zong, Yao Kang-shou, Xiong Cheng-liang

机构信息

Research Institute of Family Planning, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.

出版信息

Zhonghua Nan Ke Xue. 2011 Nov;17(11):989-92.

PMID:22141268

Abstract

OBJECTIVE

To compare the criteria of sperm morphology evaluation in the fifth edition of WHO Laboratory Manual for the Examination and Processing of Human Semen and those in the fourth edition, and to know the changes in the criteria of sperm morphology evaluation in the new edition.

METHODS

Nine technicians from Zhejiang Human Sperm Bank evaluated the morphology of 1 000 spermatozoa in 96 sperm morphological pictures according to the criteria in the fourth and fifth editions of WHO Laboratory Manual, respectively.

RESULTS

The percentage of morphologically normal sperm by the criteria of the fifth edition was (26.50 +/- 5.06)%, significantly higher than (11.39 +/- 3.17)% by the fourth edition (P < 0.05), while the rates of sperm head and tail defects based on the former were (64.26 +/- 7.66)% and (10.92 +/- 2.03)%, significantly lower than (76.11 +/- 8.18)% and (39.89 +/- 3.85)% according to the latter (P < 0.05). There were no significant differences in the rates of sperm midpiece defects and excessive residual cytoplasm by the fifth and fourth editions ([16.46 +/- 3.08]% vs [15.22 +/- 3.51 ]% and [4.24 +/- 1.66]% vs [3.87 +/- 1.68]%, P > 0.05).

CONCLUSION

The criteria of sperm morphology evaluation in the fifth edition of WHO Laboratory Manual are less strict than those in the fourth, and the percentage of morphologically normal sperm is higher according to the fifth edition.

摘要

目的

比较《世界卫生组织人类精液检查与处理实验室手册》第五版与第四版中精子形态学评估标准，了解新版精子形态学评估标准的变化。

方法

浙江人类精子库的9名技术人员分别根据《世界卫生组织人类精液检查与处理实验室手册》第四版和第五版标准，对96张精子形态学图片中的1000个精子进行形态学评估。

结果

第五版标准下形态正常精子的百分比为(26.50±5.06)%，显著高于第四版的(11.39±3.17)%(P<0.05)，而基于第五版标准的精子头部和尾部缺陷率分别为(64.26±7.66)%和(10.92±2.03)%，显著低于第四版的(76.11±8.18)%和(39.89±3.85)%(P<0.05)。第五版和第四版标准下精子中段缺陷率和过量残留细胞质率无显著差异([16.46±3.08]%对[15.22±3.51]%以及[4.24±1.66]%对[3.87±1.68]%，P>0.05)。