Helmholtz Zentrum München, Institute of Biochemical Plant Pathology, Research Unit Environmental Simulation (EUS), Ingolstädter Landstraße 1, D-85764 Neuherberg, Germany.
Karlsruhe Institute of Technology, Institute of Meteorology and Climate Research (IMK-IFU), Kreuzeckbahnstraße 19, 82467 Garmisch-Partenkirchen, Germany.
New Phytol. 2012 Apr;194(1):70-82. doi: 10.1111/j.1469-8137.2011.03979.x. Epub 2011 Dec 5.
• Depending on the atmospheric composition, isoprene emissions from plants can have a severe impact on air quality and regional climate. For the plant itself, isoprene can enhance stress tolerance and also interfere with the attraction of herbivores and parasitoids. • Here, we tested the growth performance and fitness of Populus × canescens in which isoprene emission had been knocked down by RNA interference technology (PcISPS-RNAi plants) for two growing seasons under outdoor conditions. • Neither the growth nor biomass yield of the PcISPS-RNAi poplars was impaired, and they were even temporarily enhanced compared with control poplars. Modelling of the annual carbon balances revealed a reduced carbon loss of 2.2% of the total gross primary production by the absence of isoprene emission, and a 6.9% enhanced net growth of PcISPS-RNAi poplars. However, the knock down in isoprene emission resulted in reduced susceptibility to fungal infection, whereas the attractiveness for herbivores was enhanced. • The present study promises potential for the use of non- or low-isoprene-emitting poplars for more sustainable and environmentally friendly biomass production, as reducing isoprene emission will presumably have positive effects on regional climate and air quality.
• 根据大气成分的不同，植物排放的异戊二烯会对空气质量和区域气候产生严重影响。对于植物本身来说，异戊二烯可以增强其对压力的耐受能力，同时还可以干扰其对食草动物和寄生蜂的吸引力。 • 在这里，我们在户外条件下，用 RNA 干扰技术（PcISPS-RNAi 植株）对两个生长季节的银白杨进行了实验，以测试其异戊二烯排放被敲低后的生长表现和适合度。 • PcISPS-RNAi 杨树的生长和生物量产量都没有受到损害，甚至与对照杨树相比还有暂时的增强。对年度碳平衡的模拟表明，由于不存在异戊二烯排放，总初级生产力的碳损失减少了 2.2%，PcISPS-RNAi 杨树的净生长增加了 6.9%。然而，异戊二烯排放的减少导致其对真菌感染的敏感性降低，而对食草动物的吸引力增强。 • 本研究有望为使用非或低异戊二烯排放的杨树进行更可持续和环保的生物质生产提供潜力，因为减少异戊二烯排放可能会对区域气候和空气质量产生积极影响。
