Đorđević Vukica, Stanković Đorđević Dobrila, Kocić Branislava, Dinić Marina, Sokolović Danka, Pešić Stanković Jana
Faculty of Medicine University of Niš, Serbia.
Clinical Centre of Niš, Serbia.
Oxid Med Cell Longev. 2021 Feb 25;2021:6676057. doi: 10.1155/2021/6676057. eCollection 2021.
Hepatitis C virus (HCV) is a major cause of liver disease worldwide. Chronic HCV infections are usually associated with increased oxidative stress in the liver tissue. The intensity of oxidative stress may be a detrimental factor in liver injury and may determine the severity of the disease. The aim of the present case-control study was to determine the level of lipid peroxidation (TBARS), protein oxidative modification (AOPP), and catalase activity in sera of patients infected with HCV, in relation to different HCV genotypes and viral load. Considering the HCV patients with chronic hepatitis C (52) and control subject (50) recruitment, the study was designed as a case-control-type. The HCV RNA isolation, viral load, and HCV genotyping were performed according to the standard protocols. A significant difference compared to control healthy subjects was reported for TBAR ( < 0.001), AOPP ( = 0.001), and catalase activity ( = 0.007). In a gender-based comparison, a significantly higher level of AOPP for females was reported ( < 0.001). As stratified by HCV genotype, the most common was HCV-1 (HCV-1a and HCV 1b), with the overall participation of more than 60%, followed by genotype 3, while the least represented was genotype 2. No significant difference was documented among genotypes in regard to oxidative stress markers, although somewhat higher TBARS level, but not significant, was registered in patients infected with genotype 1b. A statistically significant positive correlation was found between the concentration of HCV genome copies and AOPP ( = 0.344; = 0.012). A high level of HCV viral load was more likely to have a higher TBARS, but still without statistical significance ( = 0.072). In conclusion, the results obtained confirmed an imbalance between the ROS production and antioxidative defense system in HCV-infected patients. Since oxidative stress may have a profound influence on disease progression, fibrosis, and carcinogenesis, our results may meet the aspirations of mandatory introduction of antioxidants as early HCV therapy to counteract ROS consequences.
丙型肝炎病毒（HCV）是全球肝脏疾病的主要病因。慢性HCV感染通常与肝组织中氧化应激增加有关。氧化应激的强度可能是肝损伤的一个有害因素，并可能决定疾病的严重程度。本病例对照研究的目的是确定感染HCV患者血清中脂质过氧化水平（TBARS）、蛋白质氧化修饰水平（AOPP）和过氧化氢酶活性，以及与不同HCV基因型和病毒载量的关系。考虑到招募了慢性丙型肝炎患者（52例）和对照对象（50例），该研究设计为病例对照类型。HCV RNA分离、病毒载量和HCV基因分型均按照标准方案进行。与健康对照受试者相比，TBAR（<0.001）、AOPP（=0.001）和过氧化氢酶活性（=0.007）有显著差异。在基于性别的比较中，女性的AOPP水平显著更高（<0.001）。按HCV基因型分层，最常见的是HCV-1（HCV-1a和HCV 1b），总体占比超过60%，其次是基因型3，而代表最少的是基因型2。关于氧化应激标志物，各基因型之间未记录到显著差异，尽管感染1b基因型的患者中TBARS水平略高，但不显著。在HCV基因组拷贝浓度与AOPP之间发现了统计学上显著的正相关（=0.344；=0.012）。高HCV病毒载量更有可能具有更高的TBARS，但仍无统计学意义（=0.072）。总之，获得的结果证实了HCV感染患者中活性氧产生与抗氧化防御系统之间的失衡。由于氧化应激可能对疾病进展、纤维化和致癌作用产生深远影响，我们的结果可能符合尽早引入抗氧化剂作为HCV治疗以对抗活性氧后果的期望。
