Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Cell Mol Neurobiol. 2013 Mar;33(2):261-7. doi: 10.1007/s10571-012-9893-4. Epub 2012 Nov 29.
Methyl-3-O-methyl gallate (M3OMG) is a rare natural product that showed promising in vitro antioxidant activities. In this study, the protective role of synthetic M3OMG against sodium fluoride (NaF)-induced oxidative stress in rat brain was evaluated. Animals were treated with either M3OMG (10 and 20 mg/kg i.p.), vitamin C (10 mg/kg i.p.) as the standard antioxidant or the vehicle (5 % dimethyl sulfoxide; 1 ml/kg) for 1 week. Oxidative stress was induced in the brain by adding 600 ppm NaF in the drinking water for 7 days. At the end of the treatment period, the levels of thiobarbituric acid reactive substances (TBARS), reduced glutathione and the activities of antioxidant enzymes (superoxide dismutase and catalase) were evaluated in brain homogenates. M3OMG treatment mitigated the NaF-induced oxidative stress through normalization of the level of TBARS, reduced levels of glutathione and by the restoration of the diminished antioxidant enzyme activities. In conclusion, M3OMG could have a potential for treating neurotoxicity induced by fluoride or related environmental pollutants.
3-O-甲基没食子酸甲酯（M3OMG）是一种罕见的天然产物，具有有前景的体外抗氧化活性。在这项研究中，评估了合成的 M3OMG 对大鼠脑内氟化钠（NaF）诱导的氧化应激的保护作用。动物用 M3OMG（10 和 20 mg/kg，腹腔注射）、维生素 C（10 mg/kg，腹腔注射）作为标准抗氧化剂或载体（5%二甲基亚砜；1 ml/kg）处理 1 周。通过在饮用水中添加 600 ppm NaF 诱导 7 天来诱导脑内氧化应激。在治疗期末，评估脑匀浆中丙二醛（TBARS）、还原型谷胱甘肽的水平和抗氧化酶（超氧化物歧化酶和过氧化氢酶）的活性。M3OMG 通过使 TBARS 水平正常化、降低谷胱甘肽水平以及恢复降低的抗氧化酶活性，减轻了 NaF 诱导的氧化应激。总之，M3OMG 可能具有治疗由氟化物或相关环境污染物引起的神经毒性的潜力。
