Department of Sport Medicine, Third Faculty of Medicine, Charles University, Prague, Czech Republic.
Int J Obes (Lond). 2012 Oct;36(10):1360-5. doi: 10.1038/ijo.2011.223. Epub 2011 Dec 6.
OBJECTIVE: Hypoadiponectinemia observed in obesity is associated with insulin resistance, diabetes and atherosclerosis. The aim of the present study was to investigate secretion of adiponectin and its multimeric isoforms by explants derived from subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT) in obese and non-obese subjects. DESIGN: Paired samples of SAT and VAT and blood samples were obtained from 23 subjects (10 non-obese and 13 obese) undergoing elective abdominal surgery. Total adiponectin quantities and adiponectin isoforms were measured in conditioned media of explants derived from SAT and VAT using enzyme-linked immunosorbent assay and non-denaturing western blot, respectively. RESULTS: Total adiponectin plasma levels were lower in obese than in non-obese subjects (P<0.05). Secretion of total adiponectin in adipose tissue (AT) explants was lower in obese than in non-obese subjects in SAT (P<0.05) but not in VAT. In both, SAT and VAT, the most abundant isoform released into conditioned media was the high-molecular weight (HMW) form. Its relative proportion in relation to total adiponectin was higher in conditioned media of explants from both fat depots when compared with plasma (P<0.001). The proportion of secreted HMW vs total adiponectin was higher in VAT than in SAT explants in the group of non-obese individuals (49.3±3.1% in VAT vs 40.6±2.8% in SAT; P<0.01), whereas no difference between the two depots was found in obese subjects (46.2±3.0 % in VAT vs 46.0±2.4 % in SAT). CONCLUSION: Obesity is associated with the decrease of total adiponectin secretion in SAT. The profile of adiponectin isoforms secreted by SAT and VAT explants differs from that in plasma. Secretion of total adiponectin and HMW isoform of adiponectin are different in obese and non-obese subjects in relation to AT depot.
目的：肥胖症患者存在低脂联素血症，与胰岛素抵抗、糖尿病和动脉粥样硬化有关。本研究旨在研究肥胖和非肥胖患者皮下脂肪组织（SAT）和内脏脂肪组织（VAT）来源的脂肪组织碎片中脂联素及其多聚体同工型的分泌情况。
设计：从 23 例行择期腹部手术的患者中获取 SAT 和 VAT 配对样本和血样。使用酶联免疫吸附测定法和非变性 Western 印迹法分别检测 SAT 和 VAT 来源的组织碎片条件培养基中的总脂联素量和脂联素同工型。
结果：肥胖患者的总脂联素血浆水平低于非肥胖患者（P<0.05）。SAT 中的肥胖患者脂肪组织（AT）碎片分泌的总脂联素低于非肥胖患者（P<0.05），但 VAT 中无差异。在 SAT 和 VAT 中，释放到条件培养基中的最丰富同工型是高分子量（HMW）形式。与血浆相比，其在条件培养基中与总脂联素的相对比例更高（P<0.001）。与 SAT 相比，非肥胖个体的 VAT 来源的 HMW 与总脂联素的分泌比例更高（49.3±3.1%在 VAT 与 40.6±2.8%在 SAT；P<0.01），而肥胖患者在两个脂肪组织之间没有差异（46.2±3.0%在 VAT 与 46.0±2.4%在 SAT）。
结论：肥胖与 SAT 中总脂联素分泌减少有关。SAT 和 VAT 组织碎片分泌的脂联素同工型谱与血浆中的不同。与脂肪组织储存部位有关，肥胖和非肥胖患者的总脂联素和 HMW 脂联素同工型的分泌不同。
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