Department of Physiology, University of Ulsan College of Medicine, Seoul, South Korea.
Diabetes Res Clin Pract. 2013 Sep;101(3):341-8. doi: 10.1016/j.diabres.2013.07.002. Epub 2013 Jul 19.
To assess the importance of adipose tissue sirtuin 1 (SIRT1) in the regulation of whole-body metabolism in humans with obesity and type 2 diabetes.
In total, 19 non-diabetic obese women, 19 type 2 diabetic women undergoing gastric bypass surgery, and 27 normal-weight women undergoing gynecological surgery (total 65 women) were enrolled. Their anthropometric variables, abdominal fat distribution and metabolic parameters, serum adiponectin concentrations, and SIRT1 mRNA and protein and adiponectin mRNA expressions in visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) were measured.
SIRT1 mRNA levels in VAT and SAT were similar and these levels were suppressed in obese and type 2 diabetic women compared to normal-weight subjects. These decreases in SIRT1 expression were observed in both adipocytes and non-fat cells. There was a strong association between adipose tissue SIRT1 mRNA and protein levels. Adipose SIRT1 expression correlated inversely with HOMA-IR and other insulin resistance-related parameters. Adipose SIRT1 and adiponectin mRNA expression correlated very strongly and positively. SIRT1 mRNA level in VAT correlated inversely with visceral obesity whereas its expression in SAT correlated negatively with body mass index.
Adipose tissue SIRT1 may play a key role in the regulation of whole body metabolic homeostasis in humans. Downregulation of SIRT1 in VAT may contribute to the metabolic abnormalities that are associated with visceral obesity.
评估肥胖和 2 型糖尿病患者脂肪组织沉默调节蛋白 1（SIRT1）在调节全身代谢中的重要性。
共纳入 19 名非糖尿病肥胖女性、19 名接受胃旁路手术的 2 型糖尿病女性和 27 名接受妇科手术的正常体重女性（共 65 名女性）。测量了她们的人体测量学变量、腹部脂肪分布和代谢参数、血清脂联素浓度，以及内脏脂肪组织（VAT）和皮下脂肪组织（SAT）中的 SIRT1 mRNA 和蛋白以及脂联素 mRNA 表达。
VAT 和 SAT 中的 SIRT1 mRNA 水平相似，与正常体重受试者相比，肥胖和 2 型糖尿病女性的 SIRT1 水平受到抑制。这种 SIRT1 表达的降低发生在脂肪细胞和非脂肪细胞中。脂肪组织 SIRT1 mRNA 和蛋白水平之间存在很强的相关性。脂肪组织 SIRT1 表达与 HOMA-IR 和其他与胰岛素抵抗相关的参数呈负相关。脂肪组织 SIRT1 和脂联素 mRNA 表达呈很强的正相关。VAT 中 SIRT1 mRNA 水平与内脏肥胖呈负相关，而 SAT 中 SIRT1 表达与体重指数呈负相关。
脂肪组织 SIRT1 可能在调节人类全身代谢稳态中发挥关键作用。VAT 中 SIRT1 的下调可能导致与内脏肥胖相关的代谢异常。
