使用新型氧化铁剂通过 MRI 对多个细胞系进行标记用于细胞示踪。

Labeling of multiple cell lines using a new iron oxide agent for cell tracking by MRI.

机构信息

Imaging Research Laboratory, Robarts Research Institute, The University of Western Ontario, London, ON, Canada.

出版信息

Contrast Media Mol Imaging. 2011 Nov-Dec;6(6):514-22. doi: 10.1002/cmmi.456.

Abstract

Stem cells, cancer cells and immune cells were labeled by co-incubation with a new ultra-small iron oxide nanoparticle called Molday ION Rhodamine-B (MIRB). Iron staining, fluorescence imaging, transmission electron microscopy and flow cytometry were used to assess cell viability, function and labeling efficiency. This study has shown that MIRB can be used to label both adherent and nonadherent cell lines, with high viability and loading levels sufficient for their detection in vivo by MRI at 3 T.

摘要

干细胞、癌细胞和免疫细胞通过与一种新的超小氧化铁纳米颗粒——Molday ION Rhodamine-B（MIRB）共孵育进行标记。采用铁染色、荧光成像、透射电子显微镜和流式细胞术评估细胞活力、功能和标记效率。本研究表明，MIRB 可用于标记贴壁和非贴壁细胞系，具有高活力和足够的载药量，可在 3T MRI 下进行体内检测。