Kruglikov V D, Monakhova E V, Arkhangel'skaia I V, Vodop'ianov A S, Vodop'ianov S A, Avdeeva E P, Bozhko N V, Smolikova L M
Zh Mikrobiol Epidemiol Immunobiol. 2011 Sep-Oct(5):18-22.
Genotype characteristic and determination of serological properties of Vibrio cholerae nonO1/nonO139 strains that caused diseases in population of Rostov region from 2000 to 2009.
15 clinical strains of V. cholerae nonO1/nonO139 were studied. Serotyping was performed by using a kit of monospecific typing sera against serogroup 02-084 cholera vibrios obtained from Rostov Research Institute for Plague Control, PCR and VNTR-genotyping--by using specific primers described in scientific publications and constructed by us.
Serologic features of strains are very diverse and strains contain various combination of pathogenicity factor genes that seem to be interchangeable. Similar pattern was observed for VNTR-genotyping. Distribution of the examined strains by VNTR-genotyping did not correlate with either PCR-genotyping data or serotyping, or place and time of isolation.
The data obtained indicates a lack of general source of human infection even in the same location and time period. On the other hand, serological and genotypic features of V. cholerae nonO1/nonO139 may undergo changes in the process of staying in the macro organism or environment due to high plasticity of their genome.
对2000年至2009年在罗斯托夫地区人群中引起疾病的霍乱弧菌非O1/非O139菌株的基因型特征及血清学特性进行测定。
研究了15株霍乱弧菌非O1/非O139临床菌株。血清分型采用从罗斯托夫鼠疫防治研究所获得的针对02 - 084群霍乱弧菌的单特异性分型血清试剂盒进行，PCR和VNTR基因分型采用科学出版物中描述的及我们构建的特异性引物进行。
菌株的血清学特征非常多样，且菌株含有似乎可互换的各种致病因子基因组合。VNTR基因分型也观察到类似模式。通过VNTR基因分型对检测菌株的分布与PCR基因分型数据、血清分型或分离地点和时间均无相关性。
所获得的数据表明，即使在同一地点和时间段，人类感染也缺乏共同来源。另一方面，由于霍乱弧菌非O1/非O139基因组的高可塑性，其血清学和基因型特征在宿主体内或环境中停留过程中可能会发生变化。
