TRIB1 同源物 1 基因 rs17321515 A>G 多态性与中国毛南族和汉族人群血清脂质水平的关联。
Association of the TRIB1 tribbles homolog 1 gene rs17321515 A>G polymorphism and serum lipid levels in the Mulao and Han populations.
机构信息
Department of Cardiology, Institute of Cardiovascular Diseases, the First Affiliated Hospital, Guangxi Medical University, 22 Shuangyong Road, Nanning 530021, Guangxi, People's Republic of China.
出版信息
Lipids Health Dis. 2011 Dec 6;10:230. doi: 10.1186/1476-511X-10-230.
BACKGROUND
The association of rs17321515 single nucleotide polymorphism (SNP) near TRIB1 gene and serum lipid profiles has never been studied in the Chinese population. Therefore, the present study was undertaken to detect the association of rs17321515 SNP and several environmental factors on serum lipid levels in the Mulao and Han populations.
METHODS
A total of 639 unrelated subjects of Mulao nationality and 644 participants of Han nationality were randomly selected from our previous stratified randomized cluster samples. Genotypes of the TRIB1 rs17321515 A>G SNP were determined via polymerase chain reaction and restriction fragment length polymorphism, and then confirmed by direct sequencing.
RESULTS
Serum apolipoprotein (Apo) B levels were higher in Mulao than in Han (P < 0.05). There were no differences in the genotypic and allelic frequencies between the two ethnic groups (P > 0.05). High- and low-density lipoprotein cholesterol (HDL-C and LDL-C) levels in Han were different among the genotypes (P < 0.05 for each), the subjects with AG/GG genotypes had higher HDL-C and LDL-C levels than the subjects with AA genotype. Total cholesterol (TC), HDL-C, LDL-C, ApoA1 and ApoB levels in Han males were different among the genotypes (P < 0.05-0.001), the G carriers had higher TC, HDL-C, LDL-C, ApoA1 and ApoB levels than the G noncarriers. HDL-C levels in Mulao males were different among the genotypes (P < 0.05), the G carriers had lower HDL-C levels than the G noncarriers. Serum HDL-C and LDL-C levels in both ethnic groups and TG levels in Han were correlated with the genotypes or alleles (P < 0.05-0.01). TG and HDL-C levels in Mulao males and TG, HDL-C, LDL-C and ApoA1 levels in Han males were correlated with genotypes or alleles (P < 0.05-0.001). TG and ApoA1 levels in Han females were associated with genotypes (P < 0.05 for each). Serum lipid parameters were also associated with several environmental factors in both ethnic groups.
CONCLUSIONS
The associations of TRIB1 rs17321515 SNP and serum lipid levels are different between the Mulao and Han populations. These discrepancies might partly result from different TRIB1 gene-environmental interactions in both ethnic groups.
背景
TRIB1 基因附近 rs17321515 单核苷酸多态性（SNP）与血清脂质谱的关联在中国人中从未被研究过。因此，本研究旨在检测 rs17321515 SNP 与几种环境因素对中国壮族和汉族人群血清脂质水平的影响。
方法
从我们之前的分层随机聚类样本中随机选择了 639 名壮族无关个体和 644 名汉族参与者。通过聚合酶链反应和限制性片段长度多态性检测 TRIB1 rs17321515 A>G SNP 的基因型，然后通过直接测序进行确认。
结果
壮族人群的载脂蛋白（Apo）B 水平高于汉族（P<0.05）。两组人群的基因型和等位基因频率无差异（P>0.05）。汉族人群的高密度脂蛋白胆固醇（HDL-C）和低密度脂蛋白胆固醇（LDL-C）水平在不同基因型之间存在差异（P<0.05 每一项），AG/GG 基因型的个体比 AA 基因型的个体具有更高的 HDL-C 和 LDL-C 水平。汉族男性的总胆固醇（TC）、HDL-C、LDL-C、ApoA1 和 ApoB 水平在不同基因型之间存在差异（P<0.05-0.001），G 携带者的 TC、HDL-C、LDL-C、ApoA1 和 ApoB 水平高于 G 非携带者。壮族男性的 HDL-C 水平在不同基因型之间存在差异（P<0.05），G 携带者的 HDL-C 水平低于 G 非携带者。两组人群的血清 HDL-C 和 LDL-C 水平以及汉族人群的 TG 水平与基因型或等位基因相关（P<0.05-0.01）。壮族男性的 TG 和 HDL-C 水平以及汉族男性的 TG、HDL-C、LDL-C 和 ApoA1 水平与基因型或等位基因相关（P<0.05-0.001）。汉族女性的 TG 和 ApoA1 水平与基因型相关（P<0.05 每项）。两组人群的血清脂质参数也与多种环境因素相关。
结论
TRIB1 rs17321515 SNP 与血清脂质水平的关联在壮族和汉族人群中存在差异。这些差异可能部分归因于两组人群中不同的 TRIB1 基因-环境相互作用。
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