MADD-FOLH1 基因 rs7395662 单核苷酸多态性与多种环境因素对仫佬族和汉族人群血脂水平的影响。
Association of the rs7395662 SNP in the MADD-FOLH1 and several environmental factors with serum lipid levels in the Mulao and Han populations.
机构信息
1. Department of Cardiology, Institute of Cardiovascular Diseases, the First Affiliated Hospital, Guangxi Medical University, 22 Shuangyong Road, Nanning 530021, Guangxi, People's Republic of China.
出版信息
Int J Med Sci. 2013 Sep 1;10(11):1537-46. doi: 10.7150/ijms.6421. eCollection 2013.
BACKGROUND
The rs7395662 single nucleotide polymorphism (SNP) in the MADD-FOLH1 has been associated with serum lipid traits, but the results are inconsistent in different populations. The present study was undertaken to investigate the association of rs7395662 SNP and several environmental factors with serum lipid levels in the Guangxi Mulao and Han populations.
METHOD
A total of 721 subjects of Mulao and 727 subjects of Han Chinese were randomly selected from our previous stratified randomized samples. Genotyping of the SNP was performed by polymerase chain reaction and restriction fragment length polymorphism combined with gel electrophoresis, and confirmed by direct sequencing.
RESULTS
Serum apolipoprotein (Apo) B levels were higher in Mulao than in Han (P < 0.01). The allelic and genotypic frequencies in Han were different between males and females (P < 0.05 for each), but there was no difference between Mulao and Han or between Mulao males and females. The levels of low-density lipoprotein cholesterol (LDL-C) and ApoB in Mulao females were different among the genotypes (P < 0.05), the G allele carriers had higher LDL-C and ApoB levels than the G allele non-carriers. The levels of total cholesterol (TC), triglyceride (TG), LDL-C and ApoB in Han males and TC, TG and high-density lipoprotein cholesterol (HDL-C) in Han females were different among the genotypes (P < 0.05-0.01), the subjects with GG genotype in Han males had higher TC, TG, and ApoB and lower LDL-C levels than the subjects with AA or AG genotype, and the G allele carriers in Han females had lower TC and HDL-C levels than the G allele non-carriers. The levels of LDL-C and ApoB in Mulao females were correlated with the genotypes (P < 0.05 for each). The levels of HDL-C and ApoAI in Han males and HDL-C in Han females were correlated with genotypes (P < 0.05-0.001). Serum lipid parameters were also correlated with several environmental factors in both ethnic groups (P < 0.05-0.01).
CONCLUSION
The association of rs7395662 SNP and serum lipid levels is different between the Mulao and Han populations, and between males and females in both ethnic groups.
背景
MADD-FOLH1 中的 rs7395662 单核苷酸多态性（SNP）与血清脂质特征有关，但在不同人群中的结果不一致。本研究旨在探讨 rs7395662 SNP 与几种环境因素与广西毛南族和汉族人群血清脂质水平的关系。
方法
从我们之前的分层随机抽样中，随机选择了 721 名毛南族和 727 名汉族。通过聚合酶链反应和限制性片段长度多态性与凝胶电泳相结合的方法进行 SNP 基因分型，并通过直接测序进行确认。
结果
毛南族的载脂蛋白（Apo）B 水平高于汉族（P < 0.01）。汉族男性和女性的等位基因和基因型频率不同（P < 0.05），但毛南族和汉族或毛南族男性和女性之间没有差异。毛南族女性的低密度脂蛋白胆固醇（LDL-C）和 ApoB 水平在不同基因型之间存在差异（P < 0.05），G 等位基因携带者的 LDL-C 和 ApoB 水平高于 G 等位基因非携带者。汉族男性的总胆固醇（TC）、甘油三酯（TG）、LDL-C 和 ApoB 以及汉族女性的 TC、TG 和高密度脂蛋白胆固醇（HDL-C）在不同基因型之间存在差异（P < 0.05-0.01），汉族男性的 GG 基因型携带者的 TC、TG 和 ApoB 水平较高，而 LDL-C 水平较低，而 AA 或 AG 基因型携带者的 TC、TG 和 ApoB 水平较低，LDL-C 水平较高，汉族女性的 G 等位基因携带者的 TC 和 HDL-C 水平较低，而 G 等位基因非携带者的 TC 和 HDL-C 水平较高。毛南族女性的 LDL-C 和 ApoB 水平与基因型相关（P < 0.05）。汉族男性的 HDL-C 和 ApoAI 以及汉族女性的 HDL-C 水平与基因型相关（P < 0.05-0.001）。血清脂质参数与两个民族的几种环境因素也相关（P < 0.05-0.01）。
结论
rs7395662 SNP 与血清脂质水平的关系在毛南族和汉族之间以及两个民族的男性和女性之间存在差异。
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