Department of Cardiology, Institute of Cardiovascular Diseases, The First Affiliated Hospital, Guangxi Medical University, 22 Shuangyong Road, Nanning 530021, Guangxi, People's Republic of China.
Lipids Health Dis. 2013 Oct 25;12:156. doi: 10.1186/1476-511X-12-156.
The rs17145738 single nucleotide polymorphism (SNP) near MLX interacting protein-like/transducin (beta)-like 2 (MLXIPL/TBL2) loci is associated with serum lipid levels, but the results are inconsistent in diverse ethnic/racial groups. The current study was to investigate the association of MLXIPL/TBL2 rs17145738 SNP and several environmental factors with serum lipid profiles in the Guangxi Mulao and Han populations.
A total of 649 subjects of Mulao nationality and 712 participants of Han nationality aged 16-84 years were randomly selected from our previous stratified randomized samples. Genotyping was performed by polymerase chain reaction and restriction fragment length polymorphism combined with gel electrophoresis, and then confirmed by direct sequencing.
Serum apolipoprotein (Apo) B levels were higher in Mulao than in Han (P < 0.001). There were no significant differences in the genotypic and allelic frequencies of the MLXIPL/TBL2 rs17145738 SNP between the two ethnic groups or between males and females. The T allele carriers had higher triglyceride (TG) and ApoB levels in Mulao, and higher total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) levels in Han than the T allele non-carriers (P < 0.05 for all). Subgroup analyses showed that the T allele carriers had higher ApoB levels in both Mulao and Han females than the T allele non-carriers, but the T allele carriers had lower ApoB levels in Han males than the T allele non-carriers (P < 0.05, respectively). The T allele carriers in Han had higher TC, high-density lipoprotein cholesterol (HDL-C) levels and ApoA1/ApoB ratio and lower TG levels in males, and higher LDL-C levels and lower ApoA1/ApoB ratio in females than the T allele non-carriers (P < 0.05 for all). Serum TC levels in the combined population of the two ethnic groups and in Han, and HDL-C levels in Han males were correlated with genotypes (P < 0.05 for all). Serum lipid parameters were also correlated with several environmental factors (P < 0.05-0.01).
The association of MLXIPL/TBL2 rs17145738 SNP and serum lipid profiles is different between the Mulao and Han populations. There is a sex-specific association in the both ethnic groups.
位于 MLX 相互作用蛋白样/转导蛋白（β）样 2（MLXIPL/TBL2）基因座附近的 rs17145738 单核苷酸多态性（SNP）与血清脂质水平相关，但在不同种族/人群中的结果不一致。本研究旨在探讨 MLXIPL/TBL2 rs17145738 SNP 与几种环境因素与广西毛南族和汉族人群血清脂质谱的关系。
从我们之前的分层随机样本中随机选择了 649 名毛南族和 712 名汉族 16-84 岁的受试者。通过聚合酶链反应和限制性片段长度多态性结合凝胶电泳进行基因分型，然后通过直接测序进行确认。
毛南族的载脂蛋白（Apo）B 水平高于汉族（P<0.001）。两组之间或男女之间 MLXIPL/TBL2 rs17145738 SNP 的基因型和等位基因频率均无显著差异。在毛南族中，T 等位基因携带者的甘油三酯（TG）和 ApoB 水平较高，而在汉族中，T 等位基因携带者的总胆固醇（TC）和低密度脂蛋白胆固醇（LDL-C）水平较高（均 P<0.05）。亚组分析显示，毛南族和汉族女性的 T 等位基因携带者的 ApoB 水平均高于非携带者，但汉族男性的 T 等位基因携带者的 ApoB 水平低于非携带者（均 P<0.05）。汉族男性 T 等位基因携带者的 TC、高密度脂蛋白胆固醇（HDL-C）水平和 ApoA1/ApoB 比值较高，TG 水平较低，女性 LDL-C 水平较高，ApoA1/ApoB 比值较低（均 P<0.05）。两组人群的血清 TC 水平和汉族男性的 HDL-C 水平与基因型相关（均 P<0.05）。血清脂质参数还与几种环境因素相关（P<0.05-0.01）。
MLXIPL/TBL2 rs17145738 SNP 与血清脂质谱的关系在毛南族和汉族人群中不同。两组人群均存在性别特异性关联。
