School of Bioscience and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM, Bangi, Selangor, Malaysia.
Bioprocess Biosyst Eng. 2012 Jun;35(5):817-26. doi: 10.1007/s00449-011-0664-2. Epub 2011 Dec 7.
Rice bran (RB) and de-oiled rice bran (DRB) have been treated and used as the carbon source in acetone-butanol-ethanol (ABE) production using Clostridium saccharoperbutylacetonicum N1-4. The results showed that pretreated DRB produced more ABE than pretreated RB. Dilute sulfuric acid was the most suitable treatment method among the various pretreatment methods that were applied. The highest ABE obtained was 12.13 g/L, including 7.72 g/L of biobutanol, from sulfuric acid. The enzymatic hydrolysate of DRB (ESADRB), when treated with XAD-4 resin, resulted in an ABE productivity and yield of 0.1 g/L h and 0.44 g/g, respectively. The results also showed that the choice of pretreatment method for RB and DRB is an important factor in butanol production.
米糠（RB）和脱脂米糠（DRB）经过处理后被用作丙酮丁醇乙醇（ABE）生产的碳源，采用的菌种是产丁醇梭菌（Clostridium saccharoperbutylacetonicum N1-4）。结果表明，预处理后的 DRB 比预处理后的 RB 产生了更多的 ABE。在应用的各种预处理方法中，稀硫酸是最适合的处理方法。硫酸处理得到的最高 ABE 产量为 12.13g/L，其中包括 7.72g/L 的生物丁醇。经过 XAD-4 树脂处理的 DRB 酶解物（ESADRB），其 ABE 生产力和产率分别为 0.1g/L·h 和 0.44g/g。结果还表明，RB 和 DRB 的预处理方法的选择是丁醇生产的一个重要因素。
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