Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, Sofia, Bulgaria.
Biol Trace Elem Res. 2012 Jun;147(1-3):180-8. doi: 10.1007/s12011-011-9278-4. Epub 2011 Dec 7.
Zeolites, especially clinoptilolites, have wide application in removing heavy metals from different solutions and wastewater. The detoxification capacity of the clinoptilolite sorbent KLS-10-MA, a modified natural Bulgarian zeolite, applied as a food supplement in conditions of an ecotoxicological experiment with conventional food and lead was demonstrated for the first time. Laboratory mice, inbred imprinting control region strain, were used in a 90-day ecotoxicological experiment. Animals were divided into four experimental groups. Lead bioaccumulations in exposed and non-supplemented/supplemented with KLS-10-MA animals were compared. As additional control, healthy animals non-exposed to Pb were fed with conventional forage mixed with 12.5% KLS-10-MA. The dietary inclusion of the sorbent reduced Pb concentrations in exposed and supplemented mice by 84%, 89%, 91%, 77%, and 88% in carcass, liver, kidneys, bones, and feces, respectively. A mathematical model was proposed to outline the common trends of bone Pb bioaccumulation in exposed and non-supplemented/supplemented animals. Characteristic parameters of the kinetics of Pb concentrations were determined. Based on the model, the coefficient of absorption of Pb by gastrointestinal mucosa in the supplemented mice was found-η = 3.53% (versus η = 15% in non-supplemented ones). The present study clearly indicates that there is a realistic perspective to create a new drug based on modified natural clinoptilolites in cases of chronic heavy metal intoxication, without negatively affecting the environment.
沸石，特别是斜发沸石，在从不同溶液和废水中去除重金属方面有广泛的应用。首次证明，作为食品补充剂，改性天然保加利亚沸石 clinoptilolite KLS-10-MA 在常规食品和铅的生态毒理学实验条件下具有解毒能力。在一项为期 90 天的生态毒理学实验中，使用近交系印迹控制区品系的实验室小鼠。将动物分为四个实验组。比较了暴露组和未补充/补充 KLS-10-MA 的动物的铅生物累积量。作为额外的对照，未接触 Pb 的健康动物喂食含有 12.5% KLS-10-MA 的常规饲料。该吸附剂的膳食添加将暴露组和补充组小鼠的体内铅浓度分别降低了 84%、89%、91%、77%和 88%，在胴体、肝脏、肾脏、骨骼和粪便中。提出了一个数学模型来概述暴露和未补充/补充动物骨骼中 Pb 生物累积的共同趋势。确定了 Pb 浓度动力学的特征参数。基于该模型，发现补充组小鼠胃肠道黏膜对 Pb 的吸收率系数η=3.53%（而非补充组的η=15%）。本研究清楚地表明，在慢性重金属中毒的情况下，基于改性天然斜发沸石创造一种新药具有现实的前景，而不会对环境造成负面影响。
