Siberian Federal University, 79 Svobodnyi Ave, Krasnoyarsk, Russian Federation, 660041.
Institute of Biophysics SB RAS, Federal Research Center "Krasnoyarsk Science Center SB RAS", Akademgorodok, Krasnoyarsk, Russian Federation, 660036.
Appl Microbiol Biotechnol. 2019 Jan;103(1):225-237. doi: 10.1007/s00253-018-9460-0. Epub 2018 Oct 26.
The present study addresses the synthesis and properties of polyhydroxyalkanoates (PHA) of different composition synthesized by Cupriavidus eutrophus B-10646 using glycerol as a carbon substrate. Poly(3-hydroxybutyrate) [P(3HB)] was effectively synthesized in fed-batch culture in a 30-L fermenter on glycerol of various purification degrees, with 99.5, 99.7, and 82.1% content of the main component. Purified glycerol (99.7%) was used for 150-L pilot scale fermentation. The total biomass and P(3HB) concentration reached 110 and 85.8 g/L, respectively, after 45 h of fed-batch fermentation. An average volumetric productivity of P(3HB) was 1.83 g/(L h). The degree of crystallinity and molecular weight of P(3HB) synthesized on glycerol were lower than and temperature characteristics were the same as those of P(3HB) synthesized on sugars.
本研究针对利用甘油作为碳源合成的不同组成的聚羟基烷酸酯（PHA）进行了合成和性能研究。在 30L 发酵罐中，以不同纯度的甘油（主成分含量分别为 99.5%、99.7%和 82.1%）进行分批补料培养，有效合成了聚 3-羟基丁酸酯[P(3HB)]。使用纯度为 99.7%的甘油进行 150L 中试规模发酵，在 45h 的分批补料发酵后，生物量和 P(3HB)浓度分别达到 110g/L 和 85.8g/L。P(3HB)的比生长速率为 0.062h-1，平均体积产率为 1.83g/（L·h）。在甘油上合成的 P(3HB)的结晶度和分子量均低于在糖上合成的 P(3HB)，且其温度特性与在糖上合成的 P(3HB)相同。
