Molecular-Level Interface Research Center, Department of Chemistry, KAIST, Daejeon 305-701, Korea.
Langmuir. 2012 Jan 31;28(4):2151-5. doi: 10.1021/la203667z. Epub 2011 Dec 13.
The individual encapsulation of living cells has a great impact on the areas of single cell-based sensors and devices as well as fundamental studies in single cell-based biology. In this work, living Chlorella cells were encapsulated individually with abiological, functionalizable TiO(2), by a designed catalytic peptide that was inspired by biosilicification of diatoms in nature. The bioinspired cytocompatible reaction conditions allowed the encapsulated Chlorella cells to maintain their viability and original shapes. After formation of the TiO(2) shells, the shells were postfunctionalized by using catechol chemistry. Our work suggests a bioinspired approach to the interfacing of individual living cells with abiological materials in a controlled manner.
活细胞的个体包封对基于单细胞的传感器和设备领域以及基于单细胞的生物学的基础研究有重大影响。在这项工作中，受自然界硅藻生物矿化的启发，通过设计一种催化肽，用非生物的、功能化的 TiO(2) 对单个活的小球藻细胞进行了包封。这种受生物启发的细胞相容性反应条件允许被包封的小球藻细胞保持其活力和原始形状。形成 TiO(2) 壳后，再利用儿茶酚化学对壳进行后功能化。我们的工作为以可控的方式将单个活细胞与生物材料进行界面连接提供了一种受生物启发的方法。
