Yang Sung Ho, Choi Jinsu, Palanikumar L, Choi Eun Seong, Lee Juno, Kim Juan, Choi Insung S, Ryu Ja-Hyoung
Department of Chemistry Education , Korea National University of Education , Chungbuk 363-791 , Korea . Email:
Center for Cell-Encapsulation Research , Department of Chemistry , KAIST , Daejeon 305-701 , Korea . Email:
Chem Sci. 2015 Aug 1;6(8):4698-4703. doi: 10.1039/c5sc01225b. Epub 2015 May 18.
Formation of both mechanically durable and programmably degradable layer-by-layer (LbL) films in a biocompatible fashion has potential applications in cell therapy, tissue engineering, and drug-delivery systems, where the films are interfaced with living cells. In this work, we developed a simple but versatile method for generating cross-linked and responsively degradable LbL films, based on the thiol-exchange reaction, under highly cytocompatible conditions (aqueous solution at pH 7.4 and room temperature). The cytocompatibility of the processes was confirmed by coating individual yeast cells with the cross-linked LbL films and breaking the films on demand, while maintaining the cell viability. In addition, the processes were applied to the controlled release of an anticancer drug in the HeLa cells.
以生物相容的方式形成机械耐用且可编程降解的逐层（LbL）薄膜在细胞治疗、组织工程和药物递送系统中具有潜在应用，在这些系统中薄膜与活细胞相互作用。在这项工作中，我们基于硫醇交换反应，在高度细胞相容的条件下（pH 7.4的水溶液和室温），开发了一种简单但通用的方法来制备交联且可响应降解的LbL薄膜。通过用交联的LbL薄膜包覆单个酵母细胞并按需破坏薄膜，同时保持细胞活力，证实了该过程的细胞相容性。此外，该过程还应用于在HeLa细胞中抗癌药物的控释。
