Guo Qingwei, Sun Xiaobai, Yuan Changjin, Zhou Huijuan, Li Yanmei, Jie Gao, Jiang Guosheng
Qilu Children's Hospital of Shandong University, Jinan, PR China.
Acta Virol. 2011;55(4):311-6. doi: 10.4149/av_2011_04_311.
Epstein-Barr virus (EBV) replication and transcription activator (Rta) is an immediate-early transcription factor that mediates the switch from latent to lytic infection. DNA viruses often modulate the function of critical cell cycle proteins to maximize the efficiency of virus replication. Here we have examined the effect of Rta on cell cycle progression. Cell cycle analysis revealed that Rta induced HeLa cells in G0/G1-phase to reenter the S-phase. Analysis of the expression pattern of a key set of cell cycle regulators revealed that expression of Rta inhibited the expression of Rb and p53 and induced the expression of E2F1. These findings suggest that Rta plays an active role in redirecting HeLa cell physiology through an Rta-mediated cell cycle transformation.
爱泼斯坦-巴尔病毒（EBV）复制与转录激活因子（Rta）是一种立即早期转录因子，介导从潜伏感染到裂解感染的转变。DNA病毒常常调节关键细胞周期蛋白的功能，以最大化病毒复制效率。在此，我们研究了Rta对细胞周期进程的影响。细胞周期分析显示，Rta诱导处于G0/G1期的HeLa细胞重新进入S期。对一组关键细胞周期调节因子表达模式的分析表明，Rta的表达抑制了Rb和p53的表达，并诱导了E2F1的表达。这些发现提示，Rta通过Rta介导的细胞周期转变在改变HeLa细胞生理过程中发挥积极作用。
