阿利西定与次氯酸钠的化学相互作用。

Chemical interaction of alexidine and sodium hypochlorite.

机构信息

Department of Conservative Dentistry, Dental Research Institute, School of Dentistry, Seoul National University, Seoul, South Korea.

出版信息

J Endod. 2012 Jan;38(1):112-6. doi: 10.1016/j.joen.2011.10.003.

DOI:10.1016/j.joen.2011.10.003

PMID:22152633

Abstract

INTRODUCTION

Recent studies have reported the color change and formation of precipitates containing para-chloroaniline (PCA) after a reaction of sodium hypochlorite (NaOCl) and chlorhexidine (CHX). Alexidine (ALX), a biguanide disinfectant similar to CHX, has greater affinity for bacterial virulence factors than CHX. This study determined by electrospray ionization mass spectrometry (ESI-MS) and scanning electron microscopy (SEM) whether the chemical interaction between ALX and NaOCl results in PCA or precipitates.

METHODS

ESI-MS was performed on 4 different concentrations of ALX (1%, 0.5%, 0.25%, and 0.125%) with 4% NaOCl to detect the presence of PCA. As control groups, 1% ALX, 0.5% PCA, and a mixture of 2% CHX and 4% NaOCl were analyzed. The formation of precipitates on the dentinal surfaces of premolar root canals treated with the solutions of ALX and NaOCl (AN) or CHX and NaOCl (CN) was observed by SEM and the color change in the reaction solutions was also analyzed.

RESULTS

ESI-MS showed that the peak (mass/charge ratio = 128.026) in the PCA spectrum was not detected in any of the 4 AN solutions, whereas the peak was found in the CN solution. SEM revealed precipitates covering dentinal surfaces in the CN solution. The AN solutions produced no precipitate. The AN solutions changed in color from light yellow to transparent with decreasing ALX concentration, whereas peach-brown discoloration was observed in the CN solution.

CONCLUSIONS

The interaction of ALX and NaOCl did not produce PCA or precipitates, and the color of the reacted solution changed transparent with decreasing ALX concentration.

摘要

简介

最近的研究报告指出，次氯酸钠（NaOCl）与洗必泰（CHX）反应后，会发生颜色变化并形成含有对氯苯胺（PCA）的沉淀物。阿来西定（ALX）作为一种与 CHX 类似的双胍类消毒剂，对细菌毒力因子的亲和力大于 CHX。本研究通过电喷雾电离质谱（ESI-MS）和扫描电子显微镜（SEM）确定了 ALX 与 NaOCl 之间的化学相互作用是否会导致 PCA 或沉淀物的形成。

方法

对浓度分别为 1%、0.5%、0.25%和 0.125%的 4 种 ALX 与 4% NaOCl 进行 ESI-MS 检测，以检测 PCA 的存在。同时，还分析了浓度为 1%的 ALX、0.5%的 PCA 以及 2% CHX 和 4% NaOCl 的混合物作为对照组。通过 SEM 观察了 ALX 和 NaOCl（AN）或 CHX 和 NaOCl（CN）溶液处理的前磨牙根管牙本质表面沉淀物的形成情况，同时还分析了反应溶液的颜色变化。

结果

ESI-MS 显示，在任何一种 AN 溶液中均未检测到 PCA 图谱中的峰（质荷比=128.026），而在 CN 溶液中则检测到该峰。SEM 显示，CN 溶液中的牙本质表面覆盖有沉淀物。AN 溶液未产生沉淀物。随着 ALX 浓度的降低，AN 溶液的颜色从浅黄色变为透明，而 CN 溶液则呈现出桃褐色变色。