趋化因子对脂多糖激活的小胶质细胞中炎症物质表达的影响
[Effects of fractalkine on the expression of inflammatory substances in LPS-activated microglia cells].
作者信息
Zhu Xian-jin, Song Yan-fang, Zhang Qiu-yu, Cao Ying-ping, Xu Wei-qun, Su Dong-hui
机构信息
Department of Laboratory, The Affiliated Union Hospital, Fujian Medical University, Fujian Medical University, Fuzhou 350004, China.
出版信息
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2011 Dec;27(12):1298-300.
AIM
To investigate the effect of fractalkine on the expression of the tumor necrosis factor-α(TNF-α), interleukin-1β (IL-1β), and nitric oxide (NO) in LPS-activated murine microglia cells line N9.
METHODS
In vitro LPS-activated microglia cells were treated for 24 h in the presence of fractalkine. The level of TNF-α and IL-1β in the culture supernatants were measured by enzyme-linked immunosorbent assay (ELISA), The level of NO in the culture supernatants were quantitated by the NO test assay.
RESULTS
The concentration of TNF-α, IL-1β and NO in the culture supernatants evidently increased in LPS-activated microglia cells groups and prominently decreased by the fractalkine co-incubated.
CONCLUSION
It is thus concluded that fractalkine has neuroprotective functions by inhibiting the expression of inflammatory factor in activated microglia cells.
目的
研究趋化因子对脂多糖（LPS）激活的小鼠小胶质细胞系N9中肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）和一氧化氮（NO）表达的影响。
方法
在趋化因子存在的情况下，将体外LPS激活的小胶质细胞处理24小时。通过酶联免疫吸附测定（ELISA）测量培养上清液中TNF-α和IL-1β的水平，通过NO检测测定法定量培养上清液中NO的水平。
结果
LPS激活的小胶质细胞组中培养上清液中TNF-α、IL-1β和NO的浓度明显增加，而趋化因子共孵育后显著降低。
结论
因此得出结论，趋化因子通过抑制活化小胶质细胞中炎性因子的表达具有神经保护作用。
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