Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
Theriogenology. 2012 Mar 15;77(5):967-78. doi: 10.1016/j.theriogenology.2011.10.003. Epub 2011 Dec 6.
GnRH-stimulation tests were performed in 14 female and 14 male client-owned dogs of several breeds, before and 4 to 5 mo after gonadectomy. The aim of the study was to obtain more insight into the pituitary-gonadal axis in intact and neutered dogs and to establish reference values. Basal plasma luteinizing hormone (LH) and follicle-stimulating hormone (FSH) concentrations were increased significantly after gonadectomy in both bitches and male dogs. In both males and females ranges of the basal plasma FSH concentrations, before and after gonadectomy, did not overlap as opposed to the overlap in ranges of the basal plasma LH concentrations. Before gonadectomy basal plasma LH concentrations were lower and basal plasma FSH concentrations were higher in bitches than in male dogs. After gonadectomy these basal values did not differ significantly. GnRH administration before gonadectomy resulted in an increase in plasma LH and FSH concentrations in both genders. GnRH administration after gonadectomy produced an increase only in plasma LH concentrations in both genders, and a just significant increase in plasma FSH in castrated male dogs. GnRH administration before gonadectomy resulted in a significant increase in plasma testosterone concentration in both genders. In males ranges of basal and GnRH-stimulated plasma testosterone concentrations before and after gonadectomy did not overlap. Basal plasma estradiol concentrations were significantly higher in intact males than in castrated males and their ranges did not overlap. The basal estradiol concentrations in bitches before and after ovariectomy were not significantly different. At 120 min after GnRH administration, ranges of plasma estradiol concentration of intact and ovariectomized bitches no longer overlapped. In conclusion, basal plasma FSH concentration appears to be more reliable than basal plasma LH concentration for verification of neuter status in both male and female dogs. The basal plasma testosterone concentration appears to be reliable for verification of neuter status in male dogs. The plasma estradiol concentration at 120 min after GnRH administration can be used to discriminate between bitches with and without functional ovarian tissue.
在进行性腺切除术之前和之后的 4 到 5 个月，对 14 只雌性和 14 只雄性客户拥有的不同品种的犬进行了 GnRH 刺激试验。该研究的目的是更深入地了解未去势和去势犬的垂体-性腺轴，并建立参考值。在去势后，无论是母犬还是公犬，基础血浆黄体生成素（LH）和卵泡刺激素（FSH）浓度均显着增加。在公犬和母犬中，去势前后基础血浆 FSH 浓度范围均不重叠，而基础血浆 LH 浓度范围则重叠。在去势之前，母犬的基础血浆 LH 浓度较低，而基础血浆 FSH 浓度较高。去势后，这些基础值没有显着差异。在去势之前，GnRH 给药会导致两种性别中的血浆 LH 和 FSH 浓度增加。在去势后，GnRH 给药仅会导致两种性别中的血浆 LH 浓度增加，并且在去势的公犬中血浆 FSH 浓度仅会略有增加。在去势之前，GnRH 给药会导致两种性别中的血浆睾酮浓度显着增加。在去势之前，雄性的基础和 GnRH 刺激的血浆睾酮浓度范围不重叠。完整雄性的基础血浆雌二醇浓度明显高于去势雄性，且其范围不重叠。去势前后母犬的基础雌二醇浓度没有显着差异。在 GnRH 给药后 120 分钟，完整和去势母犬的血浆雌二醇浓度范围不再重叠。总之，在雄性和雌性犬中，基础血浆 FSH 浓度似乎比基础血浆 LH 浓度更可靠，可用于验证去势状态。基础血浆睾酮浓度似乎可用于验证雄性犬的去势状态。在 GnRH 给药后 120 分钟的血浆雌二醇浓度可用于区分具有和不具有功能性卵巢组织的母犬。
