Mendelsohn G, D'Agostino R, Eggleston J C, Baylin S B
J Clin Invest. 1979 Jun;63(6):1297-301. doi: 10.1172/JCI109424.
Recent immunohistochemical demonstration of calcitonin in rat pituitary has suggested that calcitonin, in addition to ACTH, endorphins, lipotropins, and melanocyte-stimulating hormones might be derived from a 31,000-dalton glycoprotein percursor molecule. This immunoperoxidase study demonstrates a similar distribution for beta-endorphin and ACTH immunoreactivity in human pituitary; however, the two peptides are not necessarily present in the same cells at all times. Calcitonin could not be demonstrated in human pituitary under conditions suitable for demonstration of the peptide in thyroid C cells. Weakly positive immunostaining could be obtained only with much increase in antiserum concentration and length of incubation, and higher concentrations of calcitonin were needed to abolish staining in preabsorption studies. It thus appears that the immunoreactive calcitonin in human pituitary differs from that in thyroid C cells. Likewise, we could not demonstrate immunoreactive endorphin in any developmental stage of medullary thyroid carcinoma. Our study suggests that caution should be applied in considering a physiologic role for calcitonin in the pituitary and in postulating a common peptide origin for endorphin and calcitonin in humans.
最近在大鼠垂体中进行的降钙素免疫组织化学显示，降钙素除了促肾上腺皮质激素、内啡肽、促脂素和促黑素细胞激素外，可能来源于一种31000道尔顿的糖蛋白前体分子。这项免疫过氧化物酶研究表明，β-内啡肽和促肾上腺皮质激素免疫反应性在人垂体中的分布相似；然而，这两种肽并非在所有时候都一定存在于同一细胞中。在适合于显示甲状腺C细胞中该肽的条件下，在人垂体中未能显示出降钙素。只有在抗血清浓度大幅增加和孵育时间延长的情况下，才能获得弱阳性免疫染色，并且在预吸收研究中需要更高浓度的降钙素才能消除染色。因此，人垂体中的免疫反应性降钙素似乎与甲状腺C细胞中的不同。同样，我们在甲状腺髓样癌的任何发育阶段都未能显示出免疫反应性内啡肽。我们的研究表明，在考虑降钙素在垂体中的生理作用以及假设人类内啡肽和降钙素具有共同的肽起源时应谨慎。
