Bertagna X Y, Nicholson W E, Pettengill O S, Sorenson G D, Mount C D, Orth D N
J Clin Endocrinol Metab. 1978 Dec;47(6):1390-3. doi: 10.1210/jcem-47-6-1390.
The DMS-79 continuous line of human small cell lung carcinoma cells, which produces immunoreactive (IR)-corticotropin (ACTH), -lipotropin (LPH), and -beta-endorphin (beta END), was found to produce IR-calcitonin (CT). Two major high molecular weight (HMW) forms of IR-CT were observed after gel exclusion chromatography under denaturing conditions (mol wt. approximately 7,000 and approximately 14,000), as well as a minor HMW IR-CT component (mol. wt. approximately 70,000). None of these IR-CT materials was extracted from DMS-79 medium by affinity chromatography using an ACTH antibody covalently bound to agarose. These results demonstrate ectopic production of HMW forms of CT and ACTH/LPH/beta END by human lung tumor cells in tissue culture, but do not support the existence of a common CT/ACTH/LPH/beta END precursor molecule.
人小细胞肺癌细胞系DMS - 79可产生免疫反应性（IR）促肾上腺皮质激素（ACTH）、促脂素（LPH）和β - 内啡肽（βEND），现已发现其可产生IR - 降钙素（CT）。在变性条件下进行凝胶排阻色谱分析后，观察到两种主要的高分子量（HMW）形式的IR - CT（分子量约为7000和约14000），以及一种次要的HMW IR - CT成分（分子量约为70000）。使用与琼脂糖共价结合的ACTH抗体通过亲和色谱法，未从DMS - 79培养基中提取出这些IR - CT物质。这些结果表明，在组织培养中，人肺肿瘤细胞可异位产生HMW形式的CT和ACTH/LPH/βEND，但不支持存在共同的CT/ACTH/LPH/βEND前体分子。
