一种用于定量检测过程中样品中脊髓灰质炎D抗原的快速酶联免疫吸附测定法的开发。
Development of a fast ELISA for quantifying polio D-antigen in in-process samples.
作者信息
ten Have R, Thomassen Y E, Hamzink M R J, Bakker W A M, Nijst O E M, Kersten G, Zomer G
机构信息
Unit Vaccinology, CIb, Centre for Infectious Disease Control, RIVM, National Institute of Public Health and the Environment, P.O. Box 1, 3720 BA Bilthoven, The Netherlands.
出版信息
Biologicals. 2012 Jan;40(1):84-7. doi: 10.1016/j.biologicals.2011.11.004. Epub 2011 Dec 6.
A fast ELISA was developed and qualified for analysis of polio D-antigen. The original 20 h-protocol was optimized by minimizing the total incubation time to 1 h, and by replacing the signal reagent 3,3',5,5'-tetramethylbenzidine by a chemiluminogenic signal reagent with a theoretical low intrinsic background and high dynamic range.
开发了一种快速酶联免疫吸附测定法（ELISA）并验证其可用于脊髓灰质炎D抗原分析。通过将总孵育时间缩短至1小时，并将信号试剂3,3',5,5'-四甲基联苯胺替换为具有理论低本底和高动态范围的化学发光信号试剂，对原来20小时的方案进行了优化。
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