Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur, Assam, India.
Colloids Surf B Biointerfaces. 2012 Apr 1;92:30-4. doi: 10.1016/j.colsurfb.2011.11.011. Epub 2011 Nov 17.
Polyhydroxyalkanoates (PHAs) are naturally occurring polyesters synthesized by bacteria for carbon and energy storage and it has commercial potential as bioplastic. The bacterial species Bacillus circulans MTCC 8167, isolated from crude oil contaminated soil, can efficiently produce medium chain length polyhydroxyalkanoates (P-3HB-co-3HV) from cheap carbon sources like dextrose. The molecular mass of P-3HB-co-3HV was reported as 5.1×10(4)Da with polydispersity index of 1.21 by gel permeation chromatography. In the present investigation different bacteria and fungi species were used for testing the biodegradability of the extracted polymer. The FTIR spectra of the biodegraded PHBV film showed a decrease in the peak from 1735 cm(-1) (untreated film) to 1675 cm(-1), and disappearance of a peak present in the control at 2922 cm(-1) indicating the breakdown of ester (>C=O) or O-R group and -C=H bond, respectively. From biodegradability testing, the tested microorganisms were found to have decisive contribution to the biodegradation of P-3HB-co-3HV polymer.
聚羟基烷酸酯（PHAs）是细菌为了储存碳和能量而合成的天然聚酯，具有作为生物塑料的商业潜力。从受原油污染的土壤中分离出的细菌 Bacillus circulans MTCC 8167 可以从葡萄糖等廉价碳源高效生产中链长度聚羟基烷酸酯（P-3HB-co-3HV）。凝胶渗透色谱法报告 P-3HB-co-3HV 的分子量为 5.1×10(4)Da，多分散指数为 1.21。在本研究中，使用不同的细菌和真菌种类来测试提取聚合物的生物降解性。生物降解 PHBV 薄膜的傅里叶变换红外光谱（FTIR）显示，1735 cm(-1)（未处理薄膜）处的峰降低到 1675 cm(-1)，并且在对照中存在的峰在 2922 cm(-1)处消失，表明酯（>C=O）或 O-R 基团和 -C=H 键分别断裂。从生物降解性测试来看，测试的微生物对 P-3HB-co-3HV 聚合物的生物降解具有决定性的贡献。
