Group of Microbiological Engineering and Industrial Biotechnology, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China.
Bioresour Technol. 2012 Jan;104:503-8. doi: 10.1016/j.biortech.2011.11.028. Epub 2011 Nov 25.
Cotton-based waste textiles were explored as alternative feedstock for production of bacterial cellulose (BC) by Gluconacetobacter xylinus. The cellulosic fabrics were treated with the ionic liquid (IL) 1-allyl-3-methylimidazolium chloride ([AMIM]Cl). [AMIM]Cl caused 25% inactivation of cellulase activity at a concentration as low as of 0.02 g/mL and decreased BC production during fermentation when present in concentrations higher than 0.0005 g/mL. Therefore, removal of residual IL by washing with hot water was highly beneficial to enzymatic saccharification as well as BC production. IL-treated fabrics exhibited a 5-7-fold higher enzymatic hydrolysis rate and gave a seven times larger yield of fermentable sugars than untreated fabrics. BC from cotton cloth hydrolysate was obtained at an yield of 10.8 g/L which was 83% higher than that from the culture grown on glucose-based medium. The BC from G. xylinus grown on IL-treated fabric hydrolysate had a 79% higher tensile strength than BC from glucose-based culture medium which suggests that waste cotton pretreated with [AMIM]Cl has potential to serve as a high-quality carbon source for BC production.
棉基废弃纺织品被探索作为替代原料，用于木葡糖酸醋杆菌生产细菌纤维素（BC）。纤维素织物用离子液体（IL）1-烯丙基-3-甲基咪唑氯化物([AMIM]Cl)进行处理。[AMIM]Cl 在浓度低至 0.02g/mL 时就导致纤维素酶活性失活 25%，当浓度高于 0.0005g/mL 时，会降低发酵过程中 BC 的产量。因此，用热水洗涤去除残留的 IL 对酶解和 BC 生产非常有益。经过 IL 处理的织物的酶水解速率提高了 5-7 倍，比未经处理的织物产生的可发酵糖产量高 7 倍。从棉织物水解物中获得的 BC 的产率为 10.8g/L，比在基于葡萄糖的培养基上生长时的产率高 83%。在经过 [AMIM]Cl 预处理的 IL 处理过的织物水解物上生长的 G. xylinus 产生的 BC 的拉伸强度比在基于葡萄糖的培养基中生长的 BC 高 79%，这表明经过预处理的废棉具有作为 BC 生产的高质量碳源的潜力。
