Ma Shihang, Xi Guoguo, Feng Xiangyuan, Yang Qi, Peng Zhenghong, Qiu Dong, Hu Yuqin, Zhao Xin, Cheng Lifeng, Duan Shengwen
Institute of Bast Fiber Crops/Center of Southern Economic Crops, Chinese Academy of Agricultural Sciences, Changsha, 410205, China.
Beijing National Laboratory for Molecular Sciences, Laboratory of Polymer Physics and Chemistry, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.
Sci Rep. 2025 May 28;15(1):18715. doi: 10.1038/s41598-025-02310-6.
The current study aims at the high-value utilization of ramie textile waste and explores a bio-synthetic pathway to convert waste ramie fibers into bacterial cellulose (BC). Ramie fibers were treated with commercial cellulase (C2730) and the hydrolysate was used as a base medium (RFH) for BC synthesis by fermentation. The enzymatic hydrolysis parameters were optimized by response surface methodology, yielding an optimal temperature of 40 °C, 64 h, and an enzyme dosage of 5.7%. Under these optimized conditions, the resultant yield of reducing sugars was 31.24 ± 0.37 g/L. And then the Novacetimonas hansenii HX1 strain isolated from kombucha was used for fermentation production of BC. The study found that adding yeast extract into RFH can significantly increase BC production, and 7.2 g/L BC can be produced within 7 days. The physical and chemical properties of BC were then analyzed, including Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and Thermogravimetric analysis (TGA), confirming its type I cellulose structure and good thermal stability. In particular, BC shows efficient adsorption capacity for Cu(II) ions in aqueous solution, with the highest adsorption efficiency reaching 95.62%. This research not only provides a new way to recycle textile waste, but also lays the foundation for the application of BC in the field of environmental remediation.
本研究旨在对苎麻纺织废弃物进行高值化利用，并探索一条将废弃苎麻纤维转化为细菌纤维素（BC）的生物合成途径。用商业纤维素酶（C2730）处理苎麻纤维，水解产物用作通过发酵合成BC的基础培养基（RFH）。采用响应面法优化酶解参数，得到最佳温度为40℃、时间为64 h、酶用量为5.7%。在这些优化条件下，还原糖的产量为31.24±0.37 g/L。然后，将从康普茶中分离得到的汉逊新醋杆菌HX1菌株用于BC的发酵生产。研究发现，向RFH中添加酵母提取物可显著提高BC产量，7天内可产7.2 g/L BC。随后对BC的物理和化学性质进行了分析，包括扫描电子显微镜（SEM）、傅里叶变换红外光谱（FTIR）、X射线衍射（XRD）和热重分析（TGA），证实了其I型纤维素结构和良好的热稳定性。特别是，BC对水溶液中的Cu（II）离子具有高效吸附能力，最高吸附效率达到95.62%。本研究不仅为纺织废弃物的回收利用提供了一条新途径，也为BC在环境修复领域的应用奠定了基础。
