Department of Pediatrics, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, Taiwan.
J Microbiol Immunol Infect. 2012 Apr;45(2):96-101. doi: 10.1016/j.jmii.2011.09.025. Epub 2011 Dec 11.
BACKGROUND/PURPOSE: Enterovirus 71 (EV71) infection may cause severe neurological and cardiopulmonary complications, especially in preschool children. This study is to investigate the seroprevalence and seroconversion of EV71, and the crossprotection of EV71 antibody against other enteroviruses among kindergarteners.
Overall 228 children in a public kindergarten were enrolled during two academic years, 2006 and 2007, in Taipei, Taiwan and we measured their EV71 neutralizing antibody. When the participants had herpangina; hand, foot and mouth disease (HFMD); febrile illness or respiratory symptoms, throat swabs were sampled and processed for viral culture and enterovirus real-time reverse transcriptase polymerase chain reaction (RT-PCR). Questionnaires, completed by the participants' guardians, surveyed the history of allergy and annual incidence of symptoms related to enterovirus infection.
Seropositive rates of EV71 were 20% (32/163) in 2006 and 6% (4/65) in 2007. The rate of EV71 seropositivity increased with age (p < 0.01) in 2006 but it did not differ between genders (p = 0.14). No seroconversion was observed from 2006 to 2007. Herpangina occurred in 64% of children with EV71 seropositivity and 48% of those without EV71 antibodies (p = 0.12). Non-71 enterovirus infection, confirmed by viral study, occurred in 53% (19/36) of the EV71-seropositive children and in 53% (102/192) of EV71-seronegative children (p = 0.89). No participants had EV71 infection during the study period.
EV71 did not frequently circulate in Taipei City from September 2006 to June 2008. Presence of EV71 neutralizing antibody was not associated with lower incidence of enterovirus infection caused by non-71 serotypes.
背景/目的：肠道病毒 71 型（EV71）感染可能导致严重的神经和心肺并发症，尤其是在学龄前儿童中。本研究旨在调查幼儿园儿童中 EV71 的血清流行率和血清转化率，以及 EV71 抗体对其他肠道病毒的交叉保护作用。
在 2006 年和 2007 年，我们在台湾台北市的一所公立幼儿园共招募了 228 名儿童，测量了他们的 EV71 中和抗体。当参与者出现疱疹性咽峡炎、手足口病（HFMD）、发热或呼吸道症状时，采集咽拭子并进行病毒培养和肠道病毒实时逆转录聚合酶链反应（RT-PCR）。由参与者的监护人填写的问卷调查了过敏史和与肠道病毒感染相关的年度症状发生率。
2006 年 EV71 血清阳性率为 20%（32/163），2007 年为 6%（4/65）。2006 年，EV71 血清阳性率随年龄增长而增加（p<0.01），但性别间无差异（p=0.14）。2006 年至 2007 年未观察到血清转化率。疱疹性咽峡炎发生在 64%的 EV71 血清阳性儿童和 48%的无 EV71 抗体儿童中（p=0.12）。通过病毒学研究证实的非 71 型肠道病毒感染发生在 53%（19/36）的 EV71 血清阳性儿童和 53%（102/192）的 EV71 血清阴性儿童中（p=0.89）。在研究期间，没有参与者发生 EV71 感染。
2006 年 9 月至 2008 年 6 月，台北市肠道病毒 71 型（EV71）不常流行。存在 EV71 中和抗体与非 71 型血清型引起的肠道病毒感染发生率较低无关。
