Institute of Food Science and Technology, National Taiwan University, Taipei, Taiwan.
J Ethnopharmacol. 2012 Feb 15;139(3):739-45. doi: 10.1016/j.jep.2011.11.049. Epub 2011 Dec 6.
Angelica sinensis (AS) is a Chinese herbal medicine traditionally used in prescriptions for replenishing blood and treating abnormal menstruation and other women's diseases.
This study aimed to separate and identify the major hematopoietic fraction from Angelica sinensis polysaccharides (ASPS), and to investigate the myeloprotective activity of the major bioactive fraction of ASPS as a possible supporting agent for cancer treatments.
The ASPS was fractionated with DEAE-Sepharose CL-6B column to obtain four fractions (F1, F2, F3 and F4). Each fraction was cultured with human peripheral blood mononuclear cells (MNCs) to collect conditioned medium (CM). The hematopoietic ability of various MNC-CM was then evaluated by the colony-forming assay on CD34(+) cells collected by the MACS method from human umbilical cord blood (UCB). In myeloprotective experiment, Adriblastina was used to act as the myelosuppressive agent. The monosaccharide composition of ASPS was analyzed by high-performance anion-exchange chromatography-pulse amperometric detector.
The F2 fraction, which was found to have the highest hematopoietic activity, stimulated the human peripheral blood MNCs to secret GM-CSF and IL-3. F2 could also protect the hematopoietic function of CD34(+) cells from Adriblastina. F2 occupies 19% of ASPS and contains 0.53% protein. The monosaccharide composition of F2 was arabinose (51.82%), fructose (1.65%), galactose (29.96%), glucose (4.78%) and galacturonic acid (14.80%), with molecular weight 2.5-295 kDa.
The bioactive fraction identified and fractionated from ASPS may be used as a health-promoting agent for anemia patients and cancer patients under chemoradiation treatment.
当归是一种中药，传统上用于补血和治疗月经不调及其他妇科疾病的方剂。
本研究旨在从当归多糖（ASPS）中分离和鉴定主要的造血部分，并研究 ASPS 主要生物活性部分的骨髓保护活性，作为癌症治疗的辅助剂。
ASPS 经 DEAE-Sepharose CL-6B 柱层析分离得到四个部分（F1、F2、F3 和 F4）。用各部分培养人外周血单个核细胞（MNC）收集条件培养基（CM）。然后用 MACS 法从人脐血（UCB）中收集 CD34+细胞，用集落形成试验评价各种 MNC-CM 的造血能力。在骨髓保护实验中，阿霉素作为骨髓抑制剂。用高效阴离子交换色谱-脉冲安培检测器分析 ASPS 的单糖组成。
发现具有最高造血活性的 F2 部分刺激人外周血 MNC 分泌 GM-CSF 和 IL-3。F2 还可以保护 CD34+细胞的造血功能免受阿霉素的影响。F2 占 ASPS 的 19%，含有 0.53%的蛋白质。F2 的单糖组成是阿拉伯糖（51.82%）、果糖（1.65%）、半乳糖（29.96%）、葡萄糖（4.78%）和半乳糖醛酸（14.80%），分子量为 2.5-295 kDa。
从当归中分离和鉴定的生物活性部分可能被用作贫血患者和接受放化疗的癌症患者的保健品。
