NBC Meshtec Inc., Hino, Tokyo, Japana; National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan.
Appl Environ Microbiol. 2012 Feb;78(4):951-5. doi: 10.1128/AEM.06284-11. Epub 2011 Dec 9.
We investigated the antiviral activity of nanosized copper(I) iodide (CuI) particles having an average size of 160 nm. CuI particles showed aqueous stability and generated hydroxyl radicals, which were probably derived from monovalent copper (Cu(+)). We confirmed that CuI particles showed antiviral activity against an influenza A virus of swine origin (pandemic [H1N1] 2009) by plaque titration assay. The virus titer decreased in a dose-dependent manner upon incubation with CuI particles, with the 50% effective concentration being approximately 17 μg/ml after exposure for 60 min. SDS-PAGE analysis confirmed the inactivation of the virus due to the degradation of viral proteins such as hemagglutinin and neuraminidase by CuI. Electron spin resonance (ESR) spectroscopy revealed that CuI generates hydroxyl radicals in aqueous solution, and radical production was found to be blocked by the radical scavenger N-acetylcysteine. Taken together, these findings indicate that CuI particles exert antiviral activity by generating hydroxyl radicals. Thus, CuI may be a useful material for protecting against viral attacks and may be suitable for applications such as filters, face masks, protective clothing, and kitchen cloths.
我们研究了平均粒径为 160nm 的纳米碘化亚铜 (CuI) 颗粒的抗病毒活性。CuI 颗粒具有水稳定性，并产生羟基自由基，这些自由基可能来源于一价铜 (Cu(+))。我们通过噬菌斑滴定法证实，CuI 颗粒对源自猪的甲型流感病毒（2009 年流行的 [H1N1] 病毒）具有抗病毒活性。病毒滴度在与 CuI 颗粒孵育时呈剂量依赖性下降，暴露 60 分钟后，50%有效浓度约为 17μg/ml。SDS-PAGE 分析证实，CuI 通过使病毒蛋白（如血凝素和神经氨酸酶）降解而使病毒失活。电子自旋共振 (ESR) 光谱表明，CuI 在水溶液中产生羟基自由基，自由基的产生被自由基清除剂 N-乙酰半胱氨酸所阻断。综上所述，这些结果表明 CuI 颗粒通过产生羟基自由基发挥抗病毒活性。因此，CuI 可能是一种用于预防病毒攻击的有用材料，可适用于过滤器、口罩、防护服和厨房抹布等应用。
