Bauer H, Hayami M, Stehfen-Gervinus J C
J Immunol. 1979 Mar;122(3):806-12.
Chickens and quails were immunized in parallel either i.v. or intramuscularly (i.m.) with lectin column-purified antigens from chick embryo cells that were transformed in vitro by avain sarcoma virus (ASV). After five to six injections, immunity of the animals was tested by challenge with ASV into the wing webs. Whereas tumor growth was inhibited after i.v. immunization with respect to incidence rate and time of tumor appearance, tumor growth was enhanced after i.m. injection. Animals that were injected with normal cell antigens served as controls. Spleen cells from only those animals that were immunized i.v. exerted a cytotoxic effect in vitro against ASV-transformed cells, whereas spleen cells from i.m. injected animals, in contrast, suppressed such cytotoxicity. The search for serum blocking or arming factors suggested that sera from i.m. injected animals block cellular cytotoxicity whereas sera from i.v. immunized animals render normal spleen cells cytotoxic (arming effect). The use of viruses from different subgroups and of antigens from gp85-lacking ASV-transformed cells indicates that immune effects were obtained against tumor cell surface antigens that differ from the antigen that is involved in virus neutralization (s-gp85).
将鸡和鹌鹑分别通过静脉注射（i.v.）或肌肉注射（i.m.），用来自经禽肉瘤病毒（ASV）体外转化的鸡胚细胞的凝集素柱纯化抗原进行免疫。经过五到六次注射后，通过将ASV注射到翼蹼中来检测动物的免疫力。静脉注射免疫后，肿瘤生长在发病率和肿瘤出现时间方面受到抑制，而肌肉注射后肿瘤生长增强。注射正常细胞抗原的动物作为对照。仅那些静脉注射免疫的动物的脾细胞在体外对ASV转化细胞发挥细胞毒性作用，而相比之下，肌肉注射动物的脾细胞则抑制这种细胞毒性。对血清阻断或武装因子的研究表明，肌肉注射动物的血清阻断细胞毒性，而静脉注射免疫动物的血清使正常脾细胞具有细胞毒性（武装作用）。使用来自不同亚组的病毒以及来自缺乏gp85的ASV转化细胞的抗原表明，获得了针对与病毒中和（s-gp85）所涉及的抗原不同的肿瘤细胞表面抗原的免疫效果。
