Institute for Clinical and Experimental Medicine, Department of Medicine, Videnska 1958/9, 14021 Prague 4, Czech Republic.
Nicotine Tob Res. 2012 Aug;14(8):998-1002. doi: 10.1093/ntr/ntr255. Epub 2011 Dec 9.
Using genome-wide screening, a polymorphism within the second intron of the FTO gene (rs2302673) was found to be associated with smoking habits in females. In a population-based, cross-sectional study, we analyzed three tagging FTO single-nucleotide polymorphisms (SNPs) for their association with smoking behavior.
Subjects from the Czech post-MONICA study, including 1,191 adult males (32.1% smokers) and 1,368 adult females (22.5% smokers) were included in this study. Smoking habits were obtained through questionnaire data analysis, and three FTO tagging SNPs were genotyped (rs17817449: intron 1, rs2302673: intron 2, and rs17818902: intron 3).
We detected slightly lower frequencies (p = .043) of the GG genotype of the rs17818902 SNP in males who quit smoking compared with others. However, the significance disappeared after adjusting for multiple testing. Within the entire population, or in either males or females alone, we failed to detect a significant difference between other FTO genotypes and smoking status. Also, the number of cigarettes smoked per day was independent of individual FTO genotypes in both genders.
We did not find an association between the FTO gene tagging variants and smoking status. FTO is unlikely to be a major genetic determinant of smoking status.
通过全基因组筛查，发现 FTO 基因（rs2302673）第二内含子的一个多态性与女性的吸烟习惯有关。在一项基于人群的横断面研究中，我们分析了三个与 FTO 单核苷酸多态性（SNP）相关的标签，以研究它们与吸烟行为的关系。
本研究纳入了捷克后 MONICA 研究中的 1191 名成年男性（32.1%的吸烟者）和 1368 名成年女性（22.5%的吸烟者）。吸烟习惯通过问卷调查数据分析获得，三个 FTO 标签 SNP（rs17817449：内含子 1、rs2302673：内含子 2 和 rs17818902：内含子 3）进行了基因分型。
我们发现，与其他男性相比，戒烟的男性 rs17818902 基因 SNP 的 GG 基因型频率略低（p =.043）。然而，在进行多次检验调整后，这种显著性消失了。在整个人群中，或在男性或女性中，我们都未能发现其他 FTO 基因型与吸烟状态之间存在显著差异。此外，在两性中，每天吸烟的数量与个体的 FTO 基因型无关。
我们没有发现 FTO 基因标签变异与吸烟状态之间存在关联。FTO 不太可能是吸烟状态的主要遗传决定因素。
