Division of Gastroenterology, University of Maryland School of Medicine, Sinai Hospital of Baltimore, Baltimore, MD 21215, USA.
Pancreas. 2012 May;41(4):530-4. doi: 10.1097/MPA.0b013e3182374ace.
Heat shock protein 70 (HSP70) is overexpressed in human pancreatic cancer cell lines. To determine if serum HSP70 levels are elevated in patients with pancreatic cancer and can function as a biomarker for early detection of pancreatic cancer.
Study subjects were divided into 3 groups: histologically proven pancreatic cancer (PC; n = 23), chronic pancreatitis (CP; n = 12), and matched normal control subjects (C; n = 10). Serum HSP70 levels were determined using a novel immunoelectrophoresis method developed and validated by the authors. Significance of difference between the groups was analyzed with analysis of variance (ANOVA). Receiver operating characteristic (ROC) curve analysis was performed to discriminate patients with pancreatic cancer from normal controls.
The mean ± SE serum HSP70 levels in the PC, CP, and C groups were 1.68 ± 0.083 ng/mL, 0.40 ± 0.057 ng/mL, and 0.04 ng/mL, respectively. Serum HSP70 levels in the PC group were significantly higher compared with either the CP or C groups (P < 0.01). The sensitivity and specificity of elevated serum HSP70 in the PC group was 74% and 90%, respectively.
Serum HSP70 levels are significantly increased in patients with pancreatic cancer and may be useful as an additional biomarker for the detection of pancreatic cancer.
热休克蛋白 70（HSP70）在人胰腺癌细胞系中过度表达。为了确定胰腺癌患者血清 HSP70 水平是否升高，并能否作为胰腺癌早期检测的生物标志物。
研究对象分为 3 组：组织学证实的胰腺癌（PC；n = 23）、慢性胰腺炎（CP；n = 12）和匹配的正常对照组（C；n = 10）。使用作者开发和验证的新免疫电泳法测定血清 HSP70 水平。采用方差分析（ANOVA）分析组间差异的显著性。进行受试者工作特征（ROC）曲线分析，以区分胰腺癌患者和正常对照者。
PC、CP 和 C 组的血清 HSP70 水平均值± SE 分别为 1.68±0.083ng/mL、0.40±0.057ng/mL 和 0.04ng/mL。PC 组血清 HSP70 水平明显高于 CP 或 C 组（P < 0.01）。PC 组中 HSP70 升高的敏感性和特异性分别为 74%和 90%。
胰腺癌患者血清 HSP70 水平显著升高，可作为检测胰腺癌的辅助生物标志物。
