Guelph Food Research Centre, Agriculture & Agri-Food Canada, 93 Stone Road West, Guelph, Ontario, Canada N1G 5C9.
Appl Microbiol Biotechnol. 2012 May;94(3):789-97. doi: 10.1007/s00253-011-3739-8. Epub 2011 Dec 10.
A comparative metabolomic analysis was conducted on Saccharomyces cerevisiae cells with and without patulin treatment using gas chromatography-mass spectrometry-based approach. A total of 72 metabolites were detected and compared, including 16 amino acids, 29 organic acids and alcohols, 19 sugars and sugar alcohols, 2 nucleotides, and 6 miscellaneous compounds. Principle component analysis showed a clear separation of metabolome between the cells with and without patulin treatment, and most of the identified metabolites contributed to the separation. A close examination of the identified metabolites showed an increased level of most of the free amino acids, an increased level of the intermediates in the tricarboxylic acid cycle, a higher amount of glycerol, a changed fatty acid composition, and a decreased level of cysteine and glutathione in the cells with patulin treatment. This finding indicated a slower protein synthesis rate and induced oxidative stress in the cells with patulin treatment, and provided new insights into the effect of toxic chemicals on the metabolism of organisms.
采用基于气相色谱-质谱联用的方法，对有和没有棒曲霉素处理的酿酒酵母细胞进行了比较代谢组学分析。共检测到并比较了 72 种代谢物，包括 16 种氨基酸、29 种有机酸和醇、19 种糖和糖醇、2 种核苷酸和 6 种其他化合物。主成分分析显示，有和没有棒曲霉素处理的细胞之间的代谢组明显分离，大多数鉴定出的代谢物对分离有贡献。对鉴定出的代谢物的仔细检查表明，有棒曲霉素处理的细胞中大多数游离氨基酸水平升高，三羧酸循环中间产物水平升高，甘油含量升高，脂肪酸组成发生变化，半胱氨酸和谷胱甘肽水平降低。这一发现表明，有棒曲霉素处理的细胞中蛋白质合成速率较慢，并诱导了氧化应激，为有毒化学物质对生物体代谢的影响提供了新的见解。
