Division of Environmental and Water Resources Engineering, School of Civil and Environmental Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore.
Appl Microbiol Biotechnol. 2012 Aug;95(4):1073-82. doi: 10.1007/s00253-011-3770-9. Epub 2011 Dec 9.
This study investigated the response of different-age biofilms developed on membrane surface to a chemical uncoupler 3, 3', 4', 5-tetrachlorosalicylanilide (TCS). Results showed that adenosine triphosphate (ATP) dissipation caused by TCS would promote different-age biofilms detachment, whereas chemically inhibited cellular ATP synthesis subsequently suppressed autoinducer-2 (AI-2) and extracellular polymeric substances (EPS) production. The extent of biofilm detachment was found to be closely related to AI-2-regulated EPS contents of bacteria. It was revealed that energy dissipation induced biofilm detachability was controlled by AI-2 regulated cellular communication via AI-2-mediated EPS secretion. This study would lead to a new cleaning strategy of biologically fouled membrane.
本研究考察了膜表面不同年龄生物膜对化学解偶联剂 3,3',4',5-四氯邻苯二甲酰亚胺（TCS）的反应。结果表明，TCS 引起的三磷酸腺苷（ATP）耗散会促进不同年龄生物膜的脱落，而化学抑制细胞 ATP 合成随后抑制了群体感应-2（AI-2）和胞外聚合物（EPS）的产生。生物膜脱落的程度与 AI-2 调控的细菌 EPS 含量密切相关。结果表明，能量耗散诱导的生物膜脱落可通过 AI-2 介导的 EPS 分泌调控的 AI-2 调节的细胞通讯来控制。本研究将为生物污染膜的清洗策略提供新的思路。
