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不锈钢丝网支撑的分子印迹复合膜用于从. 中选择性分离蜕皮甾酮 B

Stainless Steel Wire Mesh Supported Molecularly Imprinted Composite Membranes for Selective Separation of Ebracteolata Compound B from .

机构信息

College of Pharmacy, Qiqihar Medical University, Qiqihar 161006, China.

Department of National Immunization Program, Qiqihar Center for Disease Control and Prevention, Qiqihar 161006, China.

出版信息

Molecules. 2019 Feb 4;24(3):565. doi: 10.3390/molecules24030565.

Abstract

Stainless steel wire mesh supported molecularly imprinted composite membranes for selective separation of Ebracteolata Compound B (ECB) were prepared based on surface polymerization using ECB separated from as a template, acrylamide as a functional monomer, ethylene glycol dimethacrylate as a cross-linker, azodiisobutyronitrile as an initiator, and stainless steel wire mesh as support. Structure and purity of ECB were characterized by nuclear magenetic resonance (¹H-NMR, C-NMR) and ultra high performance liquid chromatography (UHPLC). The molecularly imprinted composite membranes were characterized by Fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM). The membrane adsorbed on the ECB reached equilibrium about 30 min later, with a maximum adsorption amount of 3.39 μmol/cm². Adsorption behavior between ECB and the molecularly imprinted composite membranes followed pseudo-second-order kinetics equation and Freundlich isotherm model. The molecularly imprinted composite membranes that could selectively identify and transport ECB in similar structures have a permeation rate of 38.71% to ECB. The ECB content in the permeation solution derived from the extract of through the imprinted membrane was 87%. Overall, the obtained results demonstrated that an efficient approach with the molecularly imprinted composite membranes for selective separation of ECB from .

摘要

基于表面聚合,以从 中分离出的埃博苷(ECB)为模板,丙烯酰胺为功能单体,乙二醇二甲基丙烯酸酯为交联剂,偶氮二异丁腈为引发剂,不锈钢丝网为支撑,制备了用于选择性分离埃博苷的不锈钢丝网支撑分子印迹复合膜。通过核磁共振(¹H-NMR、C-NMR)和超高效液相色谱(UHPLC)对 ECB 的结构和纯度进行了表征。通过傅里叶变换红外光谱(FTIR)和扫描电子显微镜(SEM)对分子印迹复合膜进行了表征。ECB 在膜上的吸附在 30 分钟后达到平衡,最大吸附量为 3.39 μmol/cm²。ECB 与分子印迹复合膜之间的吸附行为符合准二级动力学方程和弗罗因德利希等温模型。分子印迹复合膜可以选择性地识别和输送具有相似结构的 ECB,其对 ECB 的渗透速率为 38.71%。从印迹膜中提取的 的提取物中 ECB 的渗透液含量为 87%。总体而言,研究结果表明,采用分子印迹复合膜从 中选择性分离 ECB 是一种有效的方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/998a/6384690/cee4d34e7c57/molecules-24-00565-g001.jpg

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