Cappellari A, Bagarella M, Corradi G
Centro Tumori, Stabilimento Ospedaliero ULSS 8, Vicenza, Italia.
Oncology. 1990;47(5):398-400. doi: 10.1159/000226856.
In the present study the authors have examined 100 breast nodules by fine-needle aspiration cytology (FNAC) using the avidin-biotin complex method of immunoperoxidase staining. The monoclonal antibodies (MAbs) employed, B 6.2 and B 72.3, recognize two distinct molecules expressed by transformed breast epithelium. The authors have compared this immunocytochemical reaction with the conventional cytology and the histological examination as well. The data of the MAbs alone or in combination have been evaluated in terms of sensitivity, specificity, predictive value and test efficiency. The results show that tumor cells are strongly reactive with the MAbs. So the authors believe that this method in conjunction with conventional cytology may be useful in the diagnosis of breast tumor, especially in doubtful FNAC.
在本研究中，作者采用抗生物素蛋白-生物素复合物免疫过氧化物酶染色方法，通过细针穿刺细胞学检查（FNAC）对100个乳腺结节进行了检测。所使用的单克隆抗体（MAbs）B 6.2和B 72.3可识别由转化乳腺上皮细胞表达的两种不同分子。作者还将这种免疫细胞化学反应与传统细胞学及组织学检查进行了比较。单独或联合使用单克隆抗体的数据已根据敏感性、特异性、预测价值和检测效率进行了评估。结果显示，肿瘤细胞与单克隆抗体呈强反应性。因此，作者认为这种方法与传统细胞学相结合可能有助于乳腺肿瘤的诊断，尤其是在细针穿刺细胞学检查结果存疑的情况下。
