• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

利用 50MHz 电子振荡器电路开发基于石英晶体微天平（QCM）的质量敏感型 DNA 生物传感器。

Development of a mass sensitive quartz crystal microbalance (QCM)-based DNA biosensor using a 50 MHz electronic oscillator circuit.

机构信息

Dpto Tecnología Electrónica, Universidad de Vigo, Campus Lagoas Marcosende, Vigo 36310, Spain.

出版信息

Sensors (Basel). 2011;11(8):7656-64. doi: 10.3390/s110807656. Epub 2011 Aug 3.

DOI:10.3390/s110807656
PMID:22164037
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3231718/
Abstract

This work deals with the design of a high sensitivity DNA sequence detector using a 50 MHz quartz crystal microbalance (QCM) electronic oscillator circuit. The oscillator circuitry is based on Miller topology, which is able to work in damping media. Calibration and experimental study of frequency noise are carried out, finding that the designed sensor has a resolution of 7.1 ng/cm(2) in dynamic conditions (with circulation of liquid). Then the oscillator is proved as DNA biosensor. Results show that the system is able to detect the presence of complementary target DNAs in a solution with high selectivity and sensitivity. DNA target concentrations higher of 50 ng/mL can be detected.

摘要

这项工作涉及使用 50MHz 石英晶体微天平（QCM）电子振荡器电路设计高灵敏度 DNA 序列探测器。振荡器电路基于米勒拓扑结构，能够在阻尼介质中工作。对频率噪声进行校准和实验研究，发现设计的传感器在动态条件下（液体循环时）具有 7.1ng/cm²的分辨率。然后，振荡器被证明是 DNA 生物传感器。结果表明，该系统能够以高选择性和灵敏度检测溶液中互补靶 DNA 的存在。可以检测到浓度高于 50ng/ml 的 DNA 靶标。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6803/3231718/256684db23ab/sensors-11-07656f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6803/3231718/7d44841d4fb7/sensors-11-07656f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6803/3231718/99d2f0f12b2c/sensors-11-07656f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6803/3231718/b4410166a001/sensors-11-07656f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6803/3231718/9c47bfd4ca19/sensors-11-07656f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6803/3231718/256684db23ab/sensors-11-07656f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6803/3231718/7d44841d4fb7/sensors-11-07656f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6803/3231718/99d2f0f12b2c/sensors-11-07656f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6803/3231718/b4410166a001/sensors-11-07656f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6803/3231718/9c47bfd4ca19/sensors-11-07656f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6803/3231718/256684db23ab/sensors-11-07656f5.jpg

相似文献

1
Development of a mass sensitive quartz crystal microbalance (QCM)-based DNA biosensor using a 50 MHz electronic oscillator circuit.利用 50MHz 电子振荡器电路开发基于石英晶体微天平（QCM）的质量敏感型 DNA 生物传感器。
Sensors (Basel). 2011;11(8):7656-64. doi: 10.3390/s110807656. Epub 2011 Aug 3.
2
Pocketable Biosensor Based on Quartz-Crystal Microbalance and Its Application to DNA Detection.基于石英晶体微天平的口袋式生物传感器及其在 DNA 检测中的应用。
Sensors (Basel). 2022 Dec 27;23(1):281. doi: 10.3390/s23010281.
3
Frequency-shift vs phase-shift characterization of in-liquid quartz crystal microbalance applications.液体中石英晶体微天平应用的频移与相移特性
Rev Sci Instrum. 2011 Jun;82(6):064702. doi: 10.1063/1.3598340.
4
Resonance acoustic microbalance with naked-embedded quartz (RAMNE-Q) biosensor fabricated by microelectromechanical-system process.微机电系统工艺制作的裸埋石英声谐振微天平（RAMNE-Q）生物传感器。
Biosens Bioelectron. 2012 Mar 15;33(1):139-45. doi: 10.1016/j.bios.2011.12.047. Epub 2011 Dec 31.
5
An integrated dielectrophoretic quartz crystal microbalance (DEP-QCM) device for rapid biosensing applications.一种用于快速生物传感应用的集成介电泳石英晶体微天平（DEP-QCM）装置。
Biosens Bioelectron. 2007 Sep 30;23(2):225-32. doi: 10.1016/j.bios.2007.04.002. Epub 2007 Apr 6.
6
Quartz crystal microbalance (QCM) affinity biosensor for genetically modified organisms (GMOs) detection.用于转基因生物（GMOs）检测的石英晶体微天平（QCM）亲和生物传感器。
Biosens Bioelectron. 2003 Mar;18(2-3):129-40. doi: 10.1016/s0956-5663(02)00166-5.
7
Comparison of surface plasmon resonance spectroscopy and quartz crystal microbalance techniques for studying DNA assembly and hybridization.用于研究DNA组装和杂交的表面等离子体共振光谱法与石英晶体微天平技术的比较
Biosens Bioelectron. 2005 Nov 15;21(5):719-26. doi: 10.1016/j.bios.2005.01.006.
8
Immobilization, hybridization and amperometric detection of DNA sensor using quartz crystal microbalance.基于石英晶体微天平的DNA传感器的固定、杂交及安培检测
J Nanosci Nanotechnol. 2008 Sep;8(9):4553-6. doi: 10.1166/jnn.2008.ic21.
9
Replacement-free mass-amplified sandwich assay with 180-MHz electrodeless quartz-crystal microbalance biosensor.无替换物质量放大夹心测定法，采用 180MHz 无电极石英晶体微天平生物传感器。
Biosens Bioelectron. 2011 Aug 15;26(12):4819-22. doi: 10.1016/j.bios.2011.05.035. Epub 2011 May 30.
10
Preparation of Notch-4 Receptor Containing Quartz Crystal Microbalance Biosensor for MDA MB 231 Cancer Cell Detection.用于 MDA-MB-231 癌细胞检测的 Notch-4 受体含石英晶体微天平生物传感器的制备。
Methods Mol Biol. 2022;2393:515-533. doi: 10.1007/978-1-0716-1803-5_27.

引用本文的文献

1
Adsorption and Morphology Analysis of Bovine Serum Albumin on a Micropillar-Enhanced Quartz Crystal Microbalance.牛血清白蛋白在微柱增强石英晶体微天平上的吸附及形态分析。
J Phys Chem B. 2024 Oct 17;128(41):10247-10257. doi: 10.1021/acs.jpcb.4c03393. Epub 2024 Oct 9.
2
Recent Advances in Real-Time Label-Free Detection of Small Molecules.小分子实时无标记检测的最新进展
Biosensors (Basel). 2024 Feb 1;14(2):80. doi: 10.3390/bios14020080.
3
Design and development of a portable low-cost QCM-based system for liquid biosensing.用于液体生物传感的便携式低成本 QCM 系统的设计与开发。

本文引用的文献

1
Learning from nature: building bio-inspired smart nanochannels.向自然学习：构建仿生智能纳流道。
ACS Nano. 2009 Nov 24;3(11):3339-42. doi: 10.1021/nn901402b.
2
A biomimetic potassium responsive nanochannel: G-quadruplex DNA conformational switching in a synthetic nanopore.一种仿生钾响应纳米通道：合成纳米孔中的G-四链体DNA构象转换
J Am Chem Soc. 2009 Jun 10;131(22):7800-5. doi: 10.1021/ja901574c.
3
Design considerations of Miller oscillators for high-sensitivity QCM sensors in damping media.阻尼介质中用于高灵敏度石英晶体微天平传感器的米勒振荡器的设计考量
Biomed Microdevices. 2024 Jan 18;26(1):11. doi: 10.1007/s10544-024-00696-0.
4
Quantification of Particle Filtration Using a Quartz Crystal Microbalance Embedded in a Microfluidic Channel.使用嵌入微流体通道的石英晶体微天平对颗粒过滤进行定量分析。
Langmuir. 2023 Oct 10;39(40):14223-14230. doi: 10.1021/acs.langmuir.3c01331. Epub 2023 Sep 27.
5
The Impedance Analysis of a Viscoelastic Petalous Structured Stearic Acid Functional Layer Deposited on a QCM.基于 QCM 上沉积的黏弹花瓣状结构硬脂酸功能层的阻抗分析。
Sensors (Basel). 2022 Oct 3;22(19):7504. doi: 10.3390/s22197504.
6
Biosensors Based on Mechanical and Electrical Detection Techniques.基于机械和电气检测技术的生物传感器。
Sensors (Basel). 2020 Sep 30;20(19):5605. doi: 10.3390/s20195605.
7
Detection methods for influenza A H1N1 virus with special reference to biosensors: a review.甲型 H1N1 流感病毒的检测方法——特别关注生物传感器：综述。
Biosci Rep. 2020 Feb 28;40(2). doi: 10.1042/BSR20193852.
8
Recent Advances in Biosensors for Nucleic Acid and Exosome Detection.用于核酸和外泌体检测的生物传感器的最新进展
Chonnam Med J. 2019 May;55(2):86-98. doi: 10.4068/cmj.2019.55.2.86. Epub 2019 May 23.
9
Overview of Piezoelectric Biosensors, Immunosensors and DNA Sensors and Their Applications.压电生物传感器、免疫传感器和DNA传感器概述及其应用
Materials (Basel). 2018 Mar 19;11(3):448. doi: 10.3390/ma11030448.
10
Quartz Crystal Microbalance Electronic Interfacing Systems: A Review.石英晶体微天平电子接口系统：综述
Sensors (Basel). 2017 Dec 5;17(12):2799. doi: 10.3390/s17122799.
IEEE Trans Ultrason Ferroelectr Freq Control. 2007 Oct;54(10):1965-76. doi: 10.1109/tuffc.2007.490.
4
Quartz crystal microbalance (QCM) affinity biosensor for genetically modified organisms (GMOs) detection.用于转基因生物（GMOs）检测的石英晶体微天平（QCM）亲和生物传感器。
Biosens Bioelectron. 2003 Mar;18(2-3):129-40. doi: 10.1016/s0956-5663(02)00166-5.
5
Microgravimetric DNA sensor based on quartz crystal microbalance: comparison of oligonucleotide immobilization methods and the application in genetic diagnosis.基于石英晶体微天平的微重力DNA传感器：寡核苷酸固定方法的比较及其在基因诊断中的应用
Biosens Bioelectron. 2001 Jan;16(1-2):85-95. doi: 10.1016/s0956-5663(00)00136-6.
6
Validation of antibody-based recognition by piezoelectric transducers through electroacoustic admittance analysis.通过电声导纳分析验证基于抗体的压电换能器识别
Biosens Bioelectron. 1998 Mar 1;13(3-4):259-69. doi: 10.1016/s0956-5663(97)00139-5.