Ikeda I, Ishizaka Y, Tahira T, Suzuki T, Onda M, Sugimura T, Nagao M
Carcinogenesis Division, National Cancer Center Research Institute, Tokyo, Japan.
Oncogene. 1990 Sep;5(9):1291-6.
The expression of the ret proto-oncogene (proto-ret), which possibly encodes two isoforms of a receptor-type tyrosine kinase, was examined in human tumor cell lines. Expression of the proto-ret mRNA was detected in all 11 neuroblastoma cell lines examined. The level of mRNA varied more than 100-fold in these neuroblastoma cell lines and was particularly high in three of them. On the other hand, 19 non-neuroblastoma tumor cell lines derived from solid tumors and a human diploid fibroblast cell line did not express any detectable levels of proto-ret mRNA. No remarkable amplification of the proto-ret or gross structural changes in the coding region were found in these neuroblastoma cell lines. The specific expression of the proto-ret in neuroblastomas suggests that the proto-ret product may have a role in cellular functions specific to neuroblastoma cells.
原癌基因ret（proto-ret）可能编码一种受体型酪氨酸激酶的两种异构体，我们对其在人肿瘤细胞系中的表达进行了检测。在所检测的全部11种神经母细胞瘤细胞系中均检测到了proto-ret mRNA的表达。在这些神经母细胞瘤细胞系中，mRNA水平变化超过100倍，其中有三种细胞系中的表达水平特别高。另一方面，来自实体瘤的19种非神经母细胞瘤肿瘤细胞系以及一种人二倍体成纤维细胞系均未表达任何可检测水平的proto-ret mRNA。在这些神经母细胞瘤细胞系中未发现proto-ret的明显扩增或编码区的重大结构变化。proto-ret在神经母细胞瘤中的特异性表达表明，proto-ret产物可能在神经母细胞瘤细胞特有的细胞功能中发挥作用。
