Borrello M G, Bongarzone I, Pierotti M A, Luksch R, Gasparini M, Collini P, Pilotti S, Rizzetti M G, Mondellini P, De Bernardi B
Division of Experimental Oncology A, Istituto Nazionale Tumori, Milan, Italy.
Int J Cancer. 1993 Jun 19;54(4):540-5. doi: 10.1002/ijc.2910540404.
Forty-three fresh tumor specimens of human neuroblastoma belonging to different clinical stages were analyzed for the expression of 2 proto-oncogenes: trk, which encodes a tyrosine-kinase receptor for nerve growth factor (NGF) and ret, another receptor-type tyrosine kinase whose ligand is unknown. The mRNA expression of the trk gene was detected in 67.4% of cases, with increased frequency in I, II and IVs Evans' stages and in patients with favorable prognosis according to the Shimada classification. Moreover, trk expression inversely correlated with Nmyc-gene amplification. ret mRNA was found in 36.8% of cases and equally distributed in the different stages. In addition, ngfR (low-affinity NGF receptor)-gene expression was present in 9 out of 25 cases. The simultaneous presence of mRNA related to both forms of the NGF receptor, while not proving the presence of a functional receptor, indicates the existence of a sub-set of neuroblastoma cells potentially responsive to NGF.
对43例不同临床分期的人神经母细胞瘤新鲜肿瘤标本进行了分析，以检测2种原癌基因的表达：trk，它编码神经生长因子（NGF）的酪氨酸激酶受体；ret，另一种配体未知的受体型酪氨酸激酶。trk基因的mRNA表达在67.4%的病例中被检测到，在Evans分期的Ⅰ、Ⅱ和Ⅳs期以及根据岛田分类法预后良好的患者中频率增加。此外，trk表达与Nmyc基因扩增呈负相关。ret mRNA在36.8%的病例中被发现，且在不同分期中分布均匀。另外，在25例中有9例存在ngfR（低亲和力NGF受体）基因表达。与两种形式的NGF受体相关的mRNA同时存在，虽然不能证明存在功能性受体，但表明存在一组可能对NGF有反应的神经母细胞瘤细胞亚群。
