Ishizaka Y, Itoh F, Tahira T, Ikeda I, Ogura T, Sugimura T, Nagao M
Carcinogenesis Division, National Cancer Center Research Institute, Tokyo.
Jpn J Cancer Res. 1989 Dec;80(12):1149-52. doi: 10.1111/j.1349-7006.1989.tb01645.x.
In a papillary thyroid carcinoma cell line, TPC-1, we found transcripts hybridizing to ret proto-oncogene (proto-ret) cDNA probes. The transcripts hybridized to the probes encoding the kinase domain but not to those of the transmembrane and extracellular domains of proto-ret. The sizes of the main transcripts in TPC-1 were aberrant, being 2.0, 2.5, 4.0 and 5.0 kb. In the neuroblastoma cell lines, the transcripts were 3.9, 4.5, 6.0 and 7.0 kb, which have been proved to be generated by alternative splicing and polyadenylation from the non-altered proto-ret oncogene. All of the four transcripts in TPC-1 are about 2 kb smaller than the corresponding ones in the neuroblastoma. From the length of the transcripts, it is suspected that the transcripts in TPC-1 are a rearranged form of proto-ret. This is the first report describing the aberrant transcripts of proto-ret in a human tumor.
在一种甲状腺乳头状癌细胞系TPC-1中，我们发现有转录本与原癌基因ret（proto-ret）的cDNA探针杂交。这些转录本与编码激酶结构域的探针杂交，但不与proto-ret跨膜和细胞外结构域的探针杂交。TPC-1中主要转录本的大小异常，分别为2.0、2.5、4.0和5.0 kb。在神经母细胞瘤细胞系中，转录本为3.9、4.5、6.0和7.0 kb，已证明这些转录本是由未改变的原癌基因ret通过可变剪接和聚腺苷酸化产生的。TPC-1中的所有四个转录本都比神经母细胞瘤中的相应转录本小约2 kb。从转录本的长度推测，TPC-1中的转录本是proto-ret的重排形式。这是首篇描述人类肿瘤中原癌基因ret异常转录本的报告。
