Department of Restorative Sciences, University of Costa Rica, Costa Rica.
Oper Dent. 2012 Mar-Apr;37(2):172-80. doi: 10.2341/10-251-L. Epub 2011 Dec 14.
To evaluate the appearance of the hybrid layer of one total-etch and one self-etch bonding agent on human teeth with and with-out the application of 2% chlorhexidine after water storage.
Twelve human maxillary teeth (molars and premolars) had two Class II cavities (MO-OD) prepared. Teeth were separated in two groups (n=6) to receive either Adper Single Bond 2 or Adper SE Plus (3MESPE). In one cavity, the dentin donding agent was applied following manufacturer's instructions. On the adjacent cavity, 2% chlorhexidine was applied for 30 seconds before the application of the bonding agent. Teeth were sectioned mesiodistally with a slow-speed diamond disk and stored in water at 37°C for four months. The teeth were prepared for scanning electron microscope observation.The appearance of the hybrid layer was ob-served and measured by two variables: clear image of hybrid layer and presence of resintags in tubules. Data were analyzed with a Kruskal-Wallis test calculated at a 0.05 significance level.
All groups treated with chlorhexidine had the clear presence of a hybrid layer,whereas only half the specimens without chlorhexidine had a clear hybrid layer. Chlorhexidine did not affect the presence of resin tags.
The use of 2% chlorhexidine be-fore the application of a dentin-bonding agent reduced the deterioration of the hybrid layer when exposed to water.
评估在水储存后应用和不应用 2%洗必泰的情况下，一种全酸蚀和一种自酸蚀黏结剂在人牙上的混合层外观。
12 个人上颌牙齿（磨牙和前磨牙）制备了两个 II 类洞（MO-OD）。将牙齿分为两组（n=6），分别使用 Adper Single Bond 2 或 Adper SE Plus（3MESPE）。在一个窝洞，按照制造商的说明应用牙本质黏结剂。在相邻的窝洞，应用 2%洗必泰 30 秒，然后应用黏结剂。用慢速金刚石片从近远中方向将牙齿切割，在 37°C 的水中储存四个月。用扫描电子显微镜观察牙齿。通过两个变量观察和测量混合层的外观：混合层的清晰图像和管内树脂突的存在。使用 Kruskal-Wallis 检验，在 0.05 显著性水平下进行数据分析。
所有用洗必泰处理的组都清晰地存在混合层，而没有洗必泰的一半标本只有清晰的混合层。洗必泰不影响树脂突的存在。
在应用牙本质黏结剂之前使用 2%洗必泰可减少混合层在暴露于水时的恶化。
