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在实验室培养过程中出现的、具有自发孢子形成缺陷的炭疽芽孢杆菌突变体的发生、识别和回复。

Occurrence, recognition, and reversion of spontaneous, sporulation-deficient Bacillus anthracis mutants that arise during laboratory culture.

机构信息

Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892-3202, USA.

出版信息

Microbes Infect. 2012 May;14(5):387-91. doi: 10.1016/j.micinf.2011.11.009. Epub 2011 Nov 28.

Abstract

Bacillus anthracis is a spore-forming, soil-dwelling bacterium. This review describes the occurrence of spontaneous mutations leading to loss of sporulation and the selective pressures that can lead to their enrichment. We also discuss recognition of the associated phenotypes on solid medium, thereby allowing researchers to employ measures that either prevent or favor selection of sporulation-deficient mutants.

摘要

炭疽芽孢杆菌是一种形成孢子、生活在土壤中的细菌。本文综述了导致孢子形成丧失的自发突变的发生以及可能导致其富集的选择压力。我们还讨论了在固体培养基上识别相关表型的问题,从而使研究人员能够采取措施防止或有利于选择缺乏孢子形成能力的突变体。

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