丙氨酸氨基转移酶升高的抗丙型肝炎病毒反应性献血者中丙型肝炎病毒RNA的频率

Frequency of hepatitis C viral RNA in anti-hepatitis C virus non- reactive blood donors with raised alanine aminotransferase.

作者信息

Ali Nadir, Ahmed Syed Azhar, Hoch Susan Fisher, Chotani Rashid A

机构信息

Department of Haematology, Armed Forces Institute of Pathology, Rawalpindi.

出版信息

J Coll Physicians Surg Pak. 2011 Dec;21(12):782-3.

PMID:22166707

Abstract

The objective of this study was to find out the frequency of HCV RNA in anti-HCV non-reactive blood donors with raised alanine amino transferase (ALT). The study was conducted at Baqai Institute of Haematology, Baqai Medical University, Karachi, in collaboration with Combined Military Hospital, Malir Cantt, Karachi. The demographic data of blood donors was recorded, and anti-HCV, HBsAg and HIV were screened. Four hundred consecutive donors with raised ALT above the reference range were included in study. HCV RNA RT-PCR was performed on 5 sample minipools using Bio-Rad Real time PCR equipment. HCV RNA was detected in 1/400 (0.25%) blood donors. Finding of raised ALT in blood donors warrants further investigations. In case, if raised ALT is unexplained presence of HCV RNA may be suspected.

摘要

本研究的目的是查明丙氨酸氨基转移酶（ALT）升高的抗-HCV 非反应性献血者中 HCV RNA 的频率。该研究在卡拉奇巴凯医科大学巴凯血液学研究所与卡拉奇马利尔军营联合军事医院合作开展。记录了献血者的人口统计学数据，并对其进行了抗-HCV、HBsAg 和 HIV 筛查。连续纳入 400 名 ALT 高于参考范围的献血者进行研究。使用伯乐实时 PCR 设备对 5 个样本微池进行 HCV RNA RT-PCR。在 1/400（0.25%）的献血者中检测到 HCV RNA。献血者中 ALT 升高的情况值得进一步调查。如果 ALT 升高原因不明，可能怀疑存在 HCV RNA。