Department of Histology and Embryology, Faculty of Medicine, University of Trakya, Edirne, Turkey.
Food Chem Toxicol. 2012 Mar;50(3-4):719-25. doi: 10.1016/j.fct.2011.11.051. Epub 2011 Dec 6.
The aim of this study was to investigate the protective effect of quercetin (QE) on oxidative stress, apoptosis, and cell proliferation in the rat testis after streptozotocin (STZ)-induced diabetes. Diabetes was induced by a single intraperitoneal injection of STZ (50 mg/kg). The rats in the QE-treated group were given QE (15 mg/kg) once a day intraperitoneally for 8 weeks starting 3 days prior to STZ injection. At the end of the study, all animals were sacrificed. Testis tissues and blood samples were collected for histopathologic and biochemical analysis. QE treatment significantly decreased the elevated tissue malondialdehyde (MDA) levels and increased the reduced superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) enzyme activities in testis tissues samples. The QE-treated rats in the diabetic group showed an improved histologic appearance and serum testosterone levels. Our data indicate a significant reduction in the activity of in situ identification of apoptosis using terminal dUTP nick end-labeling (TUNEL) and there was a rise in the expression of proliferating cell nuclear antigen (PCNA) in testis tissues of QE-treated rats in the diabetic group. These results suggest that administration of QE is a potentially beneficial agent to reduce testicular damage in diabetic rats by decreasing oxidative stress.
本研究旨在探讨槲皮素（QE）对链脲佐菌素（STZ）诱导糖尿病大鼠睾丸氧化应激、细胞凋亡和增殖的保护作用。糖尿病通过单次腹腔注射 STZ（50mg/kg）诱导。QE 治疗组大鼠在 STZ 注射前 3 天开始每天腹腔注射 QE（15mg/kg）一次，共 8 周。研究结束时，所有动物均被处死。采集睾丸组织和血液样本进行组织病理学和生化分析。QE 治疗可显著降低睾丸组织中升高的丙二醛（MDA）水平，并增加还原型超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）酶活性。糖尿病组中接受 QE 治疗的大鼠组织学外观和血清睾酮水平得到改善。我们的数据表明，使用末端脱氧核苷酸转移酶介导的缺口末端标记（TUNEL）原位鉴定凋亡的活性显著降低，并且增殖细胞核抗原（PCNA）在糖尿病大鼠睾丸组织中的表达增加。这些结果表明，QE 的给药可能是一种通过减少氧化应激减轻糖尿病大鼠睾丸损伤的有益剂。
