Sugiyama T, Pidard D, Wautier M P, Wautier J L
INSERM U 150, Hôpital Lariboisière, Paris, France.
Nouv Rev Fr Hematol (1978). 1990;32(3):199-205.
We have studied the expression of immunochemically-related membrane proteins on human platelets and human umbilical vein endothelial cells (EC) by using cross-immunoprecipitation of 125I, surface-labeled cell extracts by cell-specific antisera, coupled to one- or two-dimensional SDS-PAGE. From 125I-labeled EC, an anti-platelet antiserum was found to immunoprecipitate major surface polypeptides comigrating with platelet GPIa, GPIIa and GPIIIa, and a protein with relative molecular mass Mr = 54,000. On the other hand, anti-EC antisera precipitated mostly GPIa, GPIc, GPIIa and GPIIIa from 125I-labeled platelets. These results supported previous reports demonstrating shared major membrane glycoproteins on platelets and EC, and suggested that the similar antigenicities in platelets and endothelial cells could play an important role in the pathogenesis of thrombosis and hemostasis in some immunologic disorders.
我们通过用细胞特异性抗血清对125I表面标记的细胞提取物进行交叉免疫沉淀，并结合一维或二维SDS-PAGE，研究了免疫化学相关膜蛋白在人血小板和人脐静脉内皮细胞（EC）上的表达。在用125I标记的EC中，发现一种抗血小板抗血清能免疫沉淀与血小板糖蛋白Ia（GPIa）、糖蛋白IIa（GPIIa）和糖蛋白IIIa（GPIIIa）共迁移的主要表面多肽，以及一种相对分子质量Mr = 54,000的蛋白质。另一方面，抗EC抗血清从125I标记的血小板中沉淀出的大多是GPIa、GPIc、GPIIa和GPIIIa。这些结果支持了先前的报道，即血小板和EC上存在共同的主要膜糖蛋白，并表明血小板和内皮细胞中相似的抗原性可能在某些免疫性疾病的血栓形成和止血发病机制中起重要作用。
