Børsum T, Hagen I, Bjerrum O J
Institute for Surgical Research, University of Oslo, Norway.
Thromb Haemost. 1987 Aug 4;58(2):686-93.
Human endothelial cells isolated from umbilical cords and cultured in primary cultures were solubilized in Triton X-100 and examined by crossed immunoelectrophoresis using rabbit antiserum against endothelial cells. Endogeneous labelling of the endothelial cell proteins with 35S-methionine or 14C-mannose followed by crossed immunoelectrophoresis and autoradiography revealed about 30 or 8 immunoprecipitates, respectively. Antigenic relationship between endothelial cell proteins and proteins in human platelets or erythrocyte membranes was demonstrated by use of the corresponding antisera and by antigen addition experiments. One of the endothelial cell proteins cross-reacted with antiserum against erythrocyte membranes and showed a partial antigenic identity reaction with the band 3 protein complex of erythrocyte membranes. The same protein showed antigenic relationship also with a platelet protein. In addition, endothelial cells contain at least 7 proteins antigenically related to platelet proteins, of which at least 5 were labelled with 14C-mannose and thus were glycoproteins. Three of these glycoproteins were antigenically related to proteins from isolated platelet membranes and three were related to the release products obtained after thrombin treatment of platelets. The present study demonstrated numerous platelet and endothelial cell proteins that were antigenically related, more than previously anticipated.
从脐带分离并在原代培养中培养的人内皮细胞，用Triton X - 100溶解，并使用抗内皮细胞兔抗血清通过交叉免疫电泳进行检测。用³⁵S - 蛋氨酸或¹⁴C - 甘露糖对内皮细胞蛋白进行内源性标记，然后进行交叉免疫电泳和放射自显影，分别显示约30种或8种免疫沉淀物。通过使用相应的抗血清和抗原添加实验，证明了内皮细胞蛋白与人血小板或红细胞膜中的蛋白之间的抗原关系。一种内皮细胞蛋白与抗红细胞膜抗血清发生交叉反应，并与红细胞膜的带3蛋白复合物显示部分抗原同一性反应。同一蛋白也与一种血小板蛋白显示抗原关系。此外，内皮细胞含有至少7种与血小板蛋白抗原相关的蛋白，其中至少5种被¹⁴C - 甘露糖标记，因此是糖蛋白。这些糖蛋白中的三种与分离的血小板膜中的蛋白抗原相关，三种与凝血酶处理血小板后获得的释放产物相关。本研究证明了许多血小板和内皮细胞蛋白具有抗原相关性，比以前预期的更多。
